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Series GSE184523 Query DataSets for GSE184523
Status Public on Mar 03, 2023
Title Murine platelet miRNA pattern and its regulation by a MK/platelet-specific genetic ablation of Dicer.
Organism Mus musculus
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary Purpose: To assess the platelet miRNA pattern which is regulated in a Dicer-dependent manner, we conducted a global screen of platelet miRNA expression of murine platelets from Dicerlox/lox and DicerPf4∆/Pf4∆ mice using Next-generation deep sequencing analysis.
Methods: Platelets were isolated from 10- to 12-week-old Dicerlox/lox and DicerPf4Δ/Pf4Δ mice of male sex. Total RNA including miRNA was isolated from murine platelets using the QIAsymphony RNA Kit. The sequencing library was prepared using the QIAseq miRNA Library Kit with 10 to 20 ng input. The libraries were pooled in an equimolar fashion and sequenced on a NovaSeq6000 as paired-end reads with a sequencing depth of >12 Mio clusters (i.e. read-pairs) per sample.
Results: we were able to detect a total of 427 miRNAs in these platelets, of which 81 were significantly regulated (> |0.5| logFC threshold) in a Dicer-dependent manner. The majority of miRNAs were significantly downregulated, but a smaller proportion of miRNAs was slightly, but significantly upregulated in platelets lacking Dicer.
Conclusions: this study discloses differential expression patterns of further platelet miRNAs that potentially link multiple intercellular processes and pathways implicated in several stages of the pathogenesis of myocardial I/R injury
Overall design Platelets miRNA profiling of 10- to 12-week-old Dicerlox/lox and DicerPf4Δ/Pf4Δ mice of male sex
Contributor(s) Schütte JP, Casadei N, Borst O
Citation(s) 36891903
Submission date Sep 21, 2021
Last update date Jun 05, 2023
Contact name Oliver Borst
Organization name University Clinic Tübingen
Department Internal Medicine III- Cardiology
Lab AG Borst
Street address Otfried-Müller-Street 10
City Tübingen
ZIP/Postal code 72076
Country Germany
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (16)
GSM5591881 platelet baseline WT rep1
GSM5591882 platelet baseline WT rep2
GSM5591883 platelet baseline WT rep3
BioProject PRJNA764916
SRA SRP338060

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Supplementary file Size Download File type/resource
GSE184523_Baseline_Dicer_lox_lox_vs_Dicer_pf4_pf4_normalized.xlsx 253.5 Kb (ftp)(http) XLSX
GSE184523_Baseline_Dicer_lox_lox_vs_Dicer_pf4_pf4_raw_data.txt.gz 18.5 Kb (ftp)(http) TXT
GSE184523_Dicer_lox_lox_Baseline_vs_Infarct.txt.gz 16.6 Kb (ftp)(http) TXT
GSE184523_Dicer_lox_lox_Baseline_vs_Infarct_normalized.xlsx 261.5 Kb (ftp)(http) XLSX
GSE184523_Dicer_pf4_pf4_Baseline_vs_Infarct_normalized.xlsx 244.3 Kb (ftp)(http) XLSX
GSE184523_Dicer_pf4_pf4_Baseline_vs_Infarct_raw_data.txt.gz 15.5 Kb (ftp)(http) TXT
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