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Series GSE184930 Query DataSets for GSE184930
Status Public on Sep 30, 2021
Title Lithium treatment and human hippocampal neurogenesis
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Lithium is a first-line treatment for bipolar disorder, where it acts as a mood-stabilizing agent. Although its precise mechanism remains unclear, neuroimaging studies have shown that lithium accumulates in the hippocampus and that chronic use amongst bipolar disorder patients is associated with larger hippocampal volumes. Here, we tested the chronic effects of low (0.75 mM) and high (2.25 mM) doses of lithium on human hippocampal progenitor cells and used immunocytochemistry to investigate the effects of lithium on cell parameters implicated in neurogenesis. Corresponding RNA-sequencing and gene-set enrichment analyses were used to evaluate whether genes affected by lithium in our model overlap with those regulating the volume of specific layers of the dentate gyrus. We observed that high-dose lithium treatment in human hippocampal progenitors increased the generation of neuroblasts (P ≤ 0.01), neurons (P ≤ 0.01), and glia (P ≤ 0.001), alongside the expression of genes which regulate the volume of the molecular layer of the dentate gyrus. This study provides empirical support that adult hippocampal neurogenesis and gliogenesis are mechanisms that could contribute to the effects of lithium on human hippocampal volume.
Overall design Transcriptomic profiling of human hippocampal progenitor cells exposed to low (0.75 mM) or high (2.25 mM) doses of lithium, and control cultures (no lithium).
Contributor(s) Palmos AB, Duarte RR, Smeeth DM, Hedges EC, Nixon DF, Thuret S, Powell TR
Citation(s) 34718328
Submission date Sep 28, 2021
Last update date Dec 02, 2021
Contact name Timothy Powell
Organization name King's College London
Department Social, Genetic & Developmental Psychiatry
Street address 16 De Crespigny Park
City London
ZIP/Postal code SE5 8AF
Country United Kingdom
Platforms (1)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
Samples (11)
GSM5600629 SB02_5-c1-6 (control)
GSM5600630 SB02_6-d1-7 (control)
GSM5600631 SB02_7-e1-5 (control)
BioProject PRJNA767056
SRA SRP339136

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Supplementary file Size Download File type/resource
GSE184930_deseq2_normalizedcounts.txt.gz 964.1 Kb (ftp)(http) TXT
GSE184930_kallisto_raw_counts.txt.gz 895.8 Kb (ftp)(http) TXT
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Processed data are available on Series record

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