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Series GSE185435 Query DataSets for GSE185435
Status Public on Mar 31, 2022
Title Chromatin reorganization during myoblast differentiation involves the caspase-dependent removal of SATB2 [ChIP-seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Induction of lineage-specific gene programs are strongly influenced by alterations in local chromatin architecture. However, key players that impact this genome reorganization remain largely unknown. Here, we report that removal of special AT-rich binding protein 2 (SATB2), a nuclear protein known to bind matrix attachment regions, is a key event in initiating myogenic differentiation. Deletion of myoblast SATB2 in vitro initiates chromatin remodeling and accelerates differentiation, while in vivo ablation depletes the muscle progenitor pool. Genome wide analysis indicates that SATB2 binding influences both chromatin loop and sub-TAD domain formation. These chromatin changes are both repressive and inductive, as loss of SATB2 leads to expression of differentiation regulatory factors and inhibition of genes that impair this process. Finally, we noted that the differentiation-specific decline in SATB2 protein is dependent on a caspase 7-mediated cleavage event. Taken together, this study demonstrates that temporal control of SATB2 protein is critical for shaping the chromatin environment and coordinating the myogenic differentiation program
Overall design Identification of Genome-wide of Satb2 binding sites in C2C12 cells.
Contributor(s) Al-Khalaf MH, Chu A, Megeney LA
Citation(s) 35326417
Submission date Oct 06, 2021
Last update date Mar 31, 2022
Contact name Lynn Megeney
Phone 6137378618
Organization name Ottawa Hospital Research Institute
Department Regenerative Medicine Program
Street address 501 Smyth Road
City Ottawa
State/province Ontario
ZIP/Postal code KIH 8L6
Country Canada
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (2)
GSM5615023 C2C12 Gr_IgG
GSM5615024 C2C12 Gr_SATB2
This SubSeries is part of SuperSeries:
GSE185437 Chromatin reorganization during myoblast differentiation involves the caspase-dependent removal of SATB2
BioProject PRJNA769207
SRA SRP340427

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Supplementary file Size Download File type/resource
GSE185435_SATB2_MACS2_Default_peaks.txt.gz 204.1 Kb (ftp)(http) TXT
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