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Series GSE185498 Query DataSets for GSE185498
Status Public on Jul 31, 2022
Title Expression of CD94 and CD96 on CD8+ T cells early after allogeneic stem cell transplantation is predictive of subsequent disease relapse and survival
Organisms Homo sapiens; Mus musculus; blank sample
Experiment type Expression profiling by high throughput sequencing
Summary Allogeneic stem cell transplantation is used widely in the treatment of hematopoietic malignancy. However relapse of malignant disease is the primary cause of treatment failure and reflects loss of the immunological graft versus leukaemia effect. We studied the transcriptional and phenotypic profile of CD8+ T cells in the first month following transplantation and related this to risk of subsequent relapse. Single cell transcriptional profiling identified 5 discrete CD8+ T cell clusters. High levels of T cell activation and acquisition of a regulatory transcriptome were apparent in patients who went on to suffer disease relapse. A relapse-associated gene signature of 39 genes was then assessed in a confirmation cohort of 34 patients. High level expression of the inhibitory receptor CD94/NKG2A on CD8+ T cells within the first month was associated with 4.8 fold increased risk of relapse and 2.7 fold reduction in survival over the subsequent 4 years. Furthermore, reduced expression of the activatory molecule CD96 was associated with 2.2 fold increased risk of relapse and 1.9 fold reduction in survival. This work identifies CD94 and CD96 as potential targets for CD8-directed immunotherapy in the very early phase following allogeneic transplantation with the potential to reduce long term relapse rates and improve patient survival.
 
Overall design RNA profiles of single peripheral CD4 and CD8 T cells at 2 weeks following allogeneic stem cell transplant
 
Contributor(s) Verma K, Croft W, Pearce H, Zu J, Stephens C, Nunnick J, Kinsella FA, Malladi R, Moss P
Citation(s) 35924575
Submission date Oct 07, 2021
Last update date Oct 30, 2022
Contact name Paul Moss
Organization name University of Birmingham
Department Immunology and Immunotherapy
Lab Moss
Street address Vincent Drive
City Birmingham
State/province West Midlands
ZIP/Postal code B152TT
Country United Kingdom
 
Platforms (3)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
GPL29487 Illumina NovaSeq 6000 (blank sample)
Samples (576)
GSM5616278 KW939_CD4_A6
GSM5616279 KW939_CD8_A6
GSM5616280 KW939_CD4_B6
Relations
BioProject PRJNA769281
SRA SRP340366

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Supplementary file Size Download File type/resource
GSE185498_raw_counts_aggregated.csv.gz 2.4 Mb (ftp)(http) CSV
GSE185498_sample_info_aggregated.csv.gz 9.9 Kb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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