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Series GSE185554 Query DataSets for GSE185554
Status Public on Oct 01, 2023
Title Single-cell Architecture and Functional Requirement of Alternative Splicing during Hematopoietic Stem Cell Emergence [RNA-Seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Hematopoietic stem cells (HSCs) are well known to generate from a rare hemogenic population of embryonic endothelial cells through a dynamic fate transition, accompanied by widespread changes in gene regulatory networks. Nevertheless, RNA splicing, a striking feature in shaping transcriptome and enabling the generation of vast transcripts and diverse proteins, has never been illustrated in this transient endothelial to HSC conversion. Here, we constructed an isoform-based transcriptome landscape during HSC development at single-cell resolution, which enables the identification of hemogenic signature isoforms, as well as stage-specific splicing events. Combining with functional screening, we showed that the inclusion of such hemogenic-specific exons was essential for hemogenic function in vitro. Moreover, their gradual appearance along with HSC specification from endothelial cells was orchestrated by the splicing regulator Srsf2. Therefore, early Srsf2 deficiency from endothelia cells dramatically impaired the HSC generation, which was accompanied by changes in the splicing pattern of several master hematopoietic regulators. These results redefine our understanding of the dynamic transcriptome diversity and demonstrate that the elaborate control of splicing governs cell fate in HSC formation.
 
Overall design Single-cell full-length RNA-sequencing of EC-associated cell populations.
 
Contributor(s) Wang F, Tan P, Zhang P, Ren Y, Zhou J, Wang D, Liu B, Lan Y, Yu J
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Submission date Oct 08, 2021
Last update date Oct 02, 2023
Contact name Jia Yu
E-mail(s) j-yu@ibms.pumc.edu.cn
Organization name Peking Union Medical College
Street address Dong Dan San Tiao 5, Dongcheng District
City Beijing
ZIP/Postal code 100005
Country China
 
Platforms (1)
GPL26624 PromethION (Mus musculus)
Samples (48)
GSM5618242 AEC10
GSM5618243 AEC11
GSM5618244 AEC12
This SubSeries is part of SuperSeries:
GSE185555 Single-cell Architecture and Functional Requirement of Alternative Splicing during Hematopoietic Stem Cell Emergence
Relations
BioProject PRJNA769638
SRA SRP340514

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE185554_RAW.tar 11.1 Mb (http)(custom) TAR (of TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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