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Series GSE186212 Query DataSets for GSE186212
Status Public on Jan 10, 2022
Title The trans-acting long noncoding RNA FLAIL represses flowering in Arabidopsis [RNA-seq]
Organism Arabidopsis thaliana
Experiment type Expression profiling by high throughput sequencing
Summary We identified differentially expressed genes in flail mutants linked to the regulation of flowering through stranded RNA-seq. A genomic rescue fragment of FLAIL (pFLAIL:gFLAIL) introduced in flail mutants can complement expression defects and early flowering, consistent with trans-acting effects of the FLAILRNA. We determined the genomic binding regions of FLAIL by ChIRP-seq. FLAIL binding to chromatin regions promotes the expression of selected flowering repressors.
Overall design RNA-seq was used to determine differentially expressed genes in wild type, flail mutant and complementation line.
Contributor(s) Jin Y, Ivanov M, Marquardt S
Citation(s) 37051749
Submission date Oct 20, 2021
Last update date Aug 25, 2023
Contact name Yu Jin
Organization name University of Copenhagen
Street address Thorvaldsensvej 40, 2nd floor
City Frederiksberg C
ZIP/Postal code 1870
Country Denmark
Platforms (1)
GPL19580 Illumina NextSeq 500 (Arabidopsis thaliana)
Samples (8)
GSM5640754 WT_RNAseq_rep1
GSM5640756 WT_RNAseq_rep2
GSM5640758 WT_RNAseq_rep3
This SubSeries is part of SuperSeries:
GSE186215 The trans-acting long noncoding RNA FLAIL represses flowering in Arabidopsis.
BioProject PRJNA772975
SRA SRP342274

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE186212_RAW.tar 441.8 Mb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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