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Status |
Public on Jan 10, 2022 |
Title |
The trans-acting long noncoding RNA FLAIL represses flowering in Arabidopsis [RNA-seq] |
Organism |
Arabidopsis thaliana |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
We identified differentially expressed genes in flail mutants linked to the regulation of flowering through stranded RNA-seq. A genomic rescue fragment of FLAIL (pFLAIL:gFLAIL) introduced in flail mutants can complement expression defects and early flowering, consistent with trans-acting effects of the FLAILRNA. We determined the genomic binding regions of FLAIL by ChIRP-seq. FLAIL binding to chromatin regions promotes the expression of selected flowering repressors.
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Overall design |
RNA-seq was used to determine differentially expressed genes in wild type, flail mutant and complementation line.
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Contributor(s) |
Jin Y, Ivanov M, Marquardt S |
Citation(s) |
37051749 |
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Submission date |
Oct 20, 2021 |
Last update date |
Aug 25, 2023 |
Contact name |
Yu Jin |
Organization name |
University of Copenhagen
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Street address |
Thorvaldsensvej 40, 2nd floor
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City |
Frederiksberg C |
ZIP/Postal code |
1870 |
Country |
Denmark |
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Platforms (1) |
GPL19580 |
Illumina NextSeq 500 (Arabidopsis thaliana) |
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Samples (8)
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This SubSeries is part of SuperSeries: |
GSE186215 |
The trans-acting long noncoding RNA FLAIL represses flowering in Arabidopsis. |
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Relations |
BioProject |
PRJNA772975 |
SRA |
SRP342274 |