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Series GSE186214 Query DataSets for GSE186214
Status Public on Feb 12, 2022
Title The trans-acting long noncoding RNA FLAIL represses flowering in Arabidopsis [ChIRP-seq]
Organism Arabidopsis thaliana
Experiment type Other
Summary We identified differentially expressed genes in flail mutants linked to the regulation of flowering through stranded RNA-seq. A genomic rescue fragment of FLAIL (pFLAIL:gFLAIL) introduced in flail mutants can complement expression defects and early flowering, consistent with trans-acting effects of the FLAILRNA. We determined the genomic binding regions of FLAIL by ChIRP-seq. FLAIL binding to chromatin regions promotes the expression of selected flowering repressors.
 
Overall design ChIRP-seq was used to determine chromatin binding regions of FLAIL.
 
Contributor(s) Jin Y, Ivanov M, Marquardt S
Citation(s) 37051749
Submission date Oct 20, 2021
Last update date Aug 25, 2023
Contact name Yu Jin
Organization name University of Copenhagen
Street address Thorvaldsensvej 40, 2nd floor
City Frederiksberg C
ZIP/Postal code 1870
Country Denmark
 
Platforms (1)
GPL19580 Illumina NextSeq 500 (Arabidopsis thaliana)
Samples (7)
GSM5640783 even_ChIRPseq_rep1
GSM5640784 even_ChIRPseq_rep2
GSM5640785 odd_ChIRPseq_rep1
This SubSeries is part of SuperSeries:
GSE186215 The trans-acting long noncoding RNA FLAIL represses flowering in Arabidopsis.
Relations
BioProject PRJNA772980
SRA SRP342275

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE186214_RAW.tar 66.5 Mb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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