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Series GSE18726 Query DataSets for GSE18726
Status Public on Oct 27, 2009
Title Astrocyte proximity modulates the myelination gene fabric of oligodendrocytes
Organism Mus musculus
Experiment type Expression profiling by array
Summary Extensive literature documented that astrocytes release neurotransmitters, cytokines and other signaling molecules that modulate migration, maturation and myelin synthesis of oligodendrocytes through mechanisms primarily converging on cytosolic [Ca2+] transients. Considering the long term effects, it is expected that astrocyte conditioned medium is a major regulator of gene expression in oligodendrocytes even in the absence of cytosol-to-cytosol communication via astrocyte-oligodendrocyte gap junction channels. Indeed, by comparing the transcriptomes of immortalized precursor oligodendrocyte (Oli-neu) cells when cultured alone and cocultured with non-touching astrocytes we found profound changes in gene expression level, control and networking. Remarkably, the astrocyte proximity was more effective in remodeling the myelination (MYE) gene fabric and its control by cytokine receptor (CYR) modulated intercellular Ca2+-signaling (ICS) transcriptomic network than the db-cAMP treatment induced transformation into myelin-associated glycoprotein-positive oligodendrocyte-like cells. Moreover, astrocyte proximity up-regulated 37 MYE genes and switched on another 14 MYE, 23 ICS and 4 CYR genes, enhancing the roles of the leukemia inhibitory factor receptor and connexins Cx29 and Cx47. The novel Prominent Gene Analysis identified enhancer of zeste homolog 2 as the most relevant MYE gene in the astrocyte proximity, notch gene homolog1 in control and B-cell leukemia/lymphoma 2 in differentiated Oli-neu cells.
 
Overall design Determine the modifications of the myelination transcriptome induced in Oli- neu control cells by differentiating treatment and proximity of cortical astrocytes. Four culture dishes of each of control, differentiated and in the astrocyte proximity Oli-neu cells were profiled using Duke mouse 30K and 36k oligonucleotide arrays in the "multiple yellow" hybrization design.
 
Contributor(s) Iacobas S, Spray DC, Iacobas DA
Citation(s) 22246408
Submission date Oct 25, 2009
Last update date Aug 04, 2016
Contact name Dumitru Andrei Iacobas
E-mail(s) daiacobas@pvamu.edu
Phone 936-261-9926
Organization name Prairie View A&M University
Department Center for Computational Systems Biology
Lab Personalized Genomics
Street address Ann Preston St
City Prairie View
State/province TX
ZIP/Postal code 77446
Country USA
 
Platforms (2)
GPL8928 Duke Mouse 36K oligonucleotide array Operon V4.0
GPL8938 Duke Mouse 30k Oligonucleotide Array Operon V3.0.1
Samples (12)
GSM465171 Oli-neu cells, control_rep1
GSM465172 Oli-neu cells, control_rep2
GSM465173 Oli-neu cells, control_rep3
Relations
BioProject PRJNA121627

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE18726_RAW.tar 38.3 Mb (http)(custom) TAR (of GPR)
Processed data included within Sample table

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