|
| Status |
Public on Jun 01, 2010 |
| Title |
Controlled expression of compatible and incompatible combinations of Ustilago maydis b-mating type locus genes bE and bW |
| Organism |
Mycosarcoma maydis |
| Experiment type |
Expression profiling by array
|
| Summary |
In the phytopathogenic Basidiomcete Ustilago maydis, sexual and pathogenic development are tightly connected and controlled via the b-mating type locus. The b-mating type locus encodes two homeodomain transcription factors, bE and bW, which form an active heterodimeric complex when they are derived from different b-alleles. To identify genes regulated by the bE/bW transcription factor, changes in gene expression were monitored during a 12-h time course (0h, 1h, 2h, 3h, 5h, 12h) in strains AB31 and AB33 that harbor the active combination bE1 and bW2 under the control of the arabinose-inducible crg1 promoter and the nitrate-inducible nar1 promoter, respectively (Brachmann et al., 2001, Mol Microbiol 42, 1047-1063). Induction of bE1/bW2 in these strains results in a filament that resembles the infectious dikaryotic hypha formed after fusion of compatible sporidia. Strains AB32 and AB34, which harbor the incompatible bE2 and bW2 combination, were used as controls.
|
| |
|
| Overall design |
Strains were grown to an OD600 of 0.4-0.6 at 28°C in liquid array medium: 6.25% (w/v) salt solution, 30 mM L-glutamine, 1% (w/v) glucose, pH 7.0 (filter-sterilized). For induction of genes under control of the crg1 promoter (strains AB31 and AB32) cells were inoculated in liquid array medium containing 1% (w/v) arabinose instead of glucose as a carbon source. For induction of the nar1-promoter glutamine was exchanged by nitrate (3g KNO3/l) as the sole nitrogen source. For induction, cells were washed once with inducing medium; time point 0 h of the timecourse experiments corresponds to the first contact with inducing medium. Experiments were performed in two biological replicates for each time point.
|
| |
|
| Contributor(s) |
Kämper J, Scherer M, Pothiratana C |
| Citation(s) |
20700446 |
| |
| Submission date |
Oct 27, 2009 |
| Last update date |
Mar 21, 2012 |
| Contact name |
Jörg Thomas Kämper |
| E-mail(s) |
joerg.kaemper@kit.edu
|
| Phone |
+49 721 608 5670
|
| Organization name |
Karlsruhe Institute of Technology
|
| Department |
Department for Applied Biosciences
|
| Street address |
Hertzstrasse 16, Geb. 06.40
|
| City |
Karlsruhe |
| ZIP/Postal code |
76187 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL3681 |
[mpiUstilagoa] MPI UstilagoA |
|
| Samples (48)
|
|
| This SubSeries is part of SuperSeries: |
| GSE18881 |
Transcription factor Rbf1 is the master regulator for b-mating type controlled pathogenic development in Ustilago maydis |
|
| Relations |
| BioProject |
PRJNA123839 |
Less...
More...