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| Status |
Public on Jul 30, 2010 |
| Title |
Gene regulation in osmpk6 mutant cells expressing OsMKK4DD |
| Organism |
Oryza sativa |
| Experiment type |
Expression profiling by array
|
| Summary |
OsMKK4 is a rice MAPKK and immediately activated by treatment with chitin elicitor, a fungal MAMP. OsMKK4 phosphrylate and activate OsMPK6. We compared gene expression in osmpk6 and osmpk6 complemented cells expressing active OsMKK4 using rice 44K oligoarray.
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| Overall design |
We used transgenic osmpk6 mutant and osmpk6 complemented callus expressing active OsMKK4 (OsMKK4DD) under the dexamethazone (DEX)-inducible promoter. Callus were treated with DEX or control ethanol. Samples were derived from 3 biological replicates and labeled by one-color method.
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| Contributor(s) |
Kaboshi MK, Takahashi A, Hirochika H |
| Citation(s) |
20525005 |
| |
| Submission date |
Oct 28, 2009 |
| Last update date |
Dec 06, 2012 |
| Contact name |
Hirohiko Hirochika |
| Organization name |
National Institute of Agrobiological Sciences
|
| Department |
Division of Genome and Biodiversity Research
|
| Street address |
2-1-2 Kannondai
|
| City |
Tsukuba |
| State/province |
Ibaraki |
| ZIP/Postal code |
305-8602 |
| Country |
Japan |
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| Platforms (1) |
| GPL6864 |
Agilent-015241 Rice Gene Expression 4x44K Microarray (Feature Number version) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA121259 |
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