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Series GSE188464 Query DataSets for GSE188464
Status Public on Nov 05, 2023
Title Disease-specific transcriptomic changes induced by NFκB inhibition in idiopathic pulmonary fibrosis
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Idiopathic pulmonary fibrosis (IPF) is a progressive fibrotic lung disease of unknown etiology and poor prognosis. In IPF, aberrant extracellular matrix production by activated, hyperproliferative fibroblasts drives disease progression but the exact mechanisms by which this occurs remains undefined. We previously showed that inhibition of transcription factor nuclear factor kappa-B (NFκB) with ACT001 reduced pro-fibrotic fibroblast behaviors such as fibroblast-to-myofibroblast transition. The aim of this follow-up study was to characterize the transcriptional profile of primary fibroblasts treated with ACT001 and identify differences between cells from patients with IPF and non-diseased control donors. Primary lung fibroblasts derived from eight patients with IPF and eight age-matched non-diseased controls (NDC) were treated with 0, 3 or 10 µM ACT001 in the presence/absence of pro-fibrotic cytokine transforming growth factor (TGF)-β1 for 24 hours after which whole genome RNA sequencing was performed. Gene set enrichment analysis (GSEA) of the Hallmark gene sets was also completed. Although the majority of genes differentially expressed after ACT001 treatment were the same in NDC and IPF cells, several genes were uniquely changed in IPF. GSEA indicated that ACT001 treatment most upregulates the reactive oxygen species pathway. ACT001 is a water-soluble compound with a stable half-life in plasma, thus making it an attractive candidate for further investigation as a therapeutic in IPF. This study adds to the growing body of literature that demonstrates anti-fibrotic activity of NF-ĸB inhibition in the context of IPF.
Overall design Primary cultered human fibroblasts from 16 individuals, 8 with idiopathic pulmonary fibrosis (IPF) and 8 age-matched controls
Contributor(s) Jaffar J, Glaspole I, Symons K, Westall G, Wong NC
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Submission date Nov 09, 2021
Last update date Nov 05, 2023
Contact name Nicholas C Wong
Organization name Monash University
Department Monash Bioinformatics Platform, Central Clinical School
Street address Wellington Road
City Clayton
State/province Victoria
ZIP/Postal code 3800
Country Australia
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (48)
GSM5683375 mRNA_45
GSM5683376 mRNA_17
GSM5683377 mRNA_157
BioProject PRJNA779019
SRA SRP345248

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Supplementary file Size Download File type/resource
GSE188464_geneCounts.txt.gz 1.5 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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