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| Status |
Public on Oct 28, 2022 |
| Title |
Genomewide m6A mapping uncovers dynamic changes in the m6A epitranscriptome of cisplatin-treated apoptotic HeLa cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We have performed a epitranscriptomics study in which we first tretaed DMSO as a negative control and cisplatin in HeLa cells. Total RNA was isolated from control as well as CP treated cells and apoptotic rate was determined by flow cytometry. Total RNAs were subjected to miCLIP to identify differentially m6A methylated RNAs. We then transcriptionally analyzed apoptotic genes which have differential m6A methylation in CP tretament by qPCR. Afterthat, candidate genes were examined at the level of translation by polysome fractioantion assay.
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| Overall design |
Total RNA was isolated from negative control (DMSO) and cisplatin treated HeLa cells in triplicate to perform miCLIP assay.
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| Contributor(s) |
Akcaoz A, Gelmez AB, Saglam B, Tuncel O, Erdogan I, Akgul B |
| Citation(s) |
36497162 |
| |
| Submission date |
Nov 10, 2021 |
| Last update date |
Jan 05, 2023 |
| Contact name |
Bünyamin Akgül |
| E-mail(s) |
bunyaminakgul@iyte.edu.tr
|
| Organization name |
Izmir Institute of Technology
|
| Department |
Molecular Biology and Genetics
|
| Lab |
Non-coding RNA Laboratory
|
| Street address |
Gulbahce Campus
|
| City |
Izmir |
| State/province |
Urla |
| ZIP/Postal code |
35433 |
| Country |
Turkey |
| |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA779444 |
| SRA |
SRP345478 |
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