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Series GSE188610 Query DataSets for GSE188610
Status Public on Jan 21, 2022
Title Two novel proteins with opposing m6A writing functions interact with HAKAI [RNA-seq]
Organism Arabidopsis thaliana
Experiment type Expression profiling by high throughput sequencing
Summary Using in vivo interaction proteomics, two HAKAI-interacting zinc finger proteins, HIZ1 and HIZ2, were discovered as novel components of the Arabidopsis m6A writer complex. HAKAI is required for the interaction between HIZ1 and MTA. Whilst HIZ1 knockout plants have normal levels of m6A, plants in which it is overexpressed show reduced methylation. In addition, HIZ1 was found to be involved in root hair development upon auxin transport inhibition in a HAKAI-dependent manner. Mutant plants lacking HIZ2 are viable but have an 85% reduction in m6A abundance and show severe developmental defects. Our findings suggest that HIZ1 appears to be a HAKAI-dependent negative regulator of m6A deposition and HIZ2 is a novel and essential member of the Arabidopsis m6A writer complex.
Overall design Differential expression analysis of Arabidopsis m6A writer complex member mutants including HAKAI, Virilizer and FIP37. We used 6 replicates for wt, 5 reps were used for fip37, 4 reps for vir and 3 for hakai. These data are related to the input data used for MeRIPSeq, which are available on GEO, accession number GSE174573.
Contributor(s) Zhang M, Bodi Z, Rataj K, Archer N, Zhong S, Mongan NP, Simpson GG, Fray RG
Citation(s) 35236848
Submission date Nov 11, 2021
Last update date Apr 22, 2022
Contact name Nathan Archer
Organization name University of Nottingham
Department School of Veterinary Medicine and Sciences
Street address Sutton Bonington
City Loughborough
State/province East Midlands
ZIP/Postal code LE12 5RD
Country United Kingdom
Platforms (1)
GPL19580 Illumina NextSeq 500 (Arabidopsis thaliana)
Samples (19)
GSM5686754 wt_1_input
GSM5686755 wt_2_input
GSM5686756 wt_3_input
This SubSeries is part of SuperSeries:
GSE188611 Two zinc finger proteins with functions in m6A writing interact with HAKAI
BioProject PRJNA779686
SRA SRP345624

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Supplementary file Size Download File type/resource
GSE188610_fip37_diffE.csv.gz 16.2 Kb (ftp)(http) CSV
GSE188610_hakai_diffE.csv.gz 3.8 Kb (ftp)(http) CSV
GSE188610_vir_diffE.csv.gz 34.9 Kb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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