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Series GSE18873 Query DataSets for GSE18873
Status Public on Nov 04, 2009
Title High throughput sequencing of RNAPII-Ser5P ChIP DNA
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The discovery of interchromosomal interactions in higher eukaryotes points to a functional interplay between genome architecture and gene expression, challenging the view of transcription as a one-dimensional process. However, the extent of interchromosomal interactions and the underlying mechanisms are unknown. Here we present the first genome-wide analysis of transcriptional interactions using the mouse globin genes in erythroid tissues. Our results show that the active globin genes associate with hundreds of other transcribed genes, revealing extensive and preferential intra- and interchromosomal transcription interactomes. We show that the transcription factor Klf1 mediates preferential co-associations of Klf1-regulated genes at a limited number of specialized transcription factories. Our results establish a new gene expression paradigm, suggesting that active co-regulated genes and their regulatory factors cooperate to create specialized nuclear hotspots optimized for efficient and coordinated transcriptional control.
 
Overall design Wild type Mus musculus RNAPII-Ser5P ChIP DNA
 
Contributor(s) Mitchell JA, Fraser P
Citation(s) 20010836
Submission date Nov 03, 2009
Last update date Jun 11, 2013
Contact name Chia-Lin Wei
E-mail(s) weicl@gis.a-star.edu.sg
Phone 65 64788074
Fax 65 64789059
URL http://www.gis.a-star.edu.sg/internet/site/investigators.php?f=cv&user_id=9
Organization name Genome Institute of Singapore
Department Genome Technology & Biology
Street address 60 Biopolis Street #02-01
City Singapore
ZIP/Postal code 138672
Country Singapore
 
Platforms (1)
GPL9522 454 GS 20 (Mus musculus)
Samples (1)
GSM467771 RNAPII-Ser5P ChIP from WT Mus musculus
Relations
BioProject PRJNA121127

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE18873_RAW.tar 49.0 Mb (http)(custom) TAR (of FNA, TXT)
Raw data provided as supplementary file
Processed data provided as supplementary file

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