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Series GSE189228 Query DataSets for GSE189228
Status Public on Dec 01, 2023
Title Establishment of a 3D hyperuricemia model based on cultured human liver organoids
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Hyperuricemia (HUA) is a metabolic disease caused by abnormal purine metabolism, the prevalence of which has increased worldwide. Here, a 3D organoid culture system for mimicking HUA in vitro was established using cultured human liver organoids. Liver organoids can be generated from single hepatocytes and passaged for several months, retaining key morphological features, functional purine metabolism and global gene expression profile. Furthermore, organoids can be differentiated into hepatocytes with high expression of maturation markers including HNF4α, E-cadherin, and ALB. Importantly, organoids can produce high level of uric acid after xanthine induction which is the substrate of xanthine oxidase. Furthermore, the preclinical application potential of this organoid model was verified by measuring the anti-hyperuricemic effect of the widely used allopurinol, as well as the reported bioactive substance puerarin. The results demonstrate that this novel organoid model could be used for high-throughput screening of both chemical and food-derived compounds with antihyperuricemic bioactivity.
Overall design Primary Hepatocytes were isolated from paracarcinomatous liver tissues by two-step collagenase digestion, and suspended the cells in Matrigel. The mRNA profile of human liver organoids after 5 days of differentiation and amplification. Total RNA was extracted from 3 liver-organoids from different passages in expansion medium (EM) and differentiation medium (DM) (P4, P6, P8) using TRIzol reagent. RNA quality and quantity using was analyzed by NanoDrop spectrophotometer (BioRad, USA) and a Bioanalyzer 2100 system (Agilent, USA). Transcriptional sequencing library was constructed by TruSeq™ RNA Sample Preparation Kit (Illumina, USA). The libraries were then sequenced on an Illumina Hiseq platform. Sequencing data analysis was performed using the DESeq2 package (CIT) in the R software environment. HEM represents organoids cultured in EM, and HDM represents organoids cultured in DM.
Contributor(s) Yao M, Ren J, Hou C, Hu Y
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Submission date Nov 19, 2021
Last update date Dec 01, 2023
Contact name Maojin Yao
Phone 8619924786386
Organization name Guangzhou Medical University
Department Guangzhou Institute of Respiratory Health
Lab State Key Laboratory of Respiratory Disease
Street address No.195, West Dongfeng Road, Yuexiu District
City Guangzhou
State/province Guangdong
ZIP/Postal code 510180
Country China
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (6)
GSM5696331 HEM_5_T0706P4
GSM5696332 HEM_5_T0706P6
GSM5696333 HEM_5_T0706P8
BioProject PRJNA782010
SRA SRP346968

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Supplementary file Size Download File type/resource
GSE189228_Genelist.txt.gz 2.5 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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