|
| Status |
Public on Sep 21, 2005 |
| Title |
Murine fast-twitch muscle: two-week progression of denervation atrophy |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
The right legs of 3 months old CD1 mice were denervated by unilaterally cutting the sciatic nerve at the level of trochanter, resulting in a permanent denervation of the lower hindleg. At the indicated times, animals were killed by cervical dislocation and tibialis anterior (TA) muscles were dissected out and frozen in liquid nitrogen. The expression analysis was carried out at five time points, namely just after denervation (time 0) and 1, 3, 7 and 14 days after nerve interruption. The contralateral, left TA muscle from the same individual served as innervated control. We reversed labeling of denervated and contralateral samples in each experiment, thus carrying out two separate hybridizations for each time point.
Keywords = mouse
Keywords = fast-twitch muscle
Keywords = denervation
Keywords = atrophy
Keywords: time-course
|
| |
|
| |
|
| Contributor(s) |
Raffaello A, Laveder P, Romualdi C, Toniolo L, Germinario E, Danieli D, Megighian A, Reggiani C, Lanfranchi G |
| Citation(s) |
16380408 |
| |
| Submission date |
Oct 27, 2004 |
| Last update date |
Jul 26, 2013 |
| Contact name |
Paolo Laveder |
| E-mail(s) |
laveder@cribi.unipd.it
|
| Phone |
0039 0498276221
|
| Fax |
0039 0498276280
|
| URL |
http://muscle.cribi.unipd.it/microarrays/index.html
|
| Organization name |
CRIBI Biotechnology Center
|
| Department |
Department of Biology
|
| Lab |
The Genomic Research, University of Padua (G.R.U.P.)
|
| Street address |
via U.Bassi 58/B
|
| City |
Padova |
| ZIP/Postal code |
35121 |
| Country |
Italy |
| |
|
| Platforms (1) |
|
| Samples (10)
|
| GSM33584 |
Denervation atrophy: expression at time 0 |
| GSM33585 |
Denervation atrophy: expression at time 0 (reverse labeling) |
| GSM33586 |
Denervation atrophy: expression at day 1 |
| GSM33587 |
Denervation atrophy: expression at day 1 (reverse labeling) |
| GSM33588 |
Denervation atrophy: expression at day 3 |
| GSM33589 |
Denervation atrophy: expression at day 3 (reverse labeling) |
| GSM33590 |
Denervation atrophy: expression at day 7 |
| GSM33591 |
Denervation atrophy: expression at day 7 (reverse labeling) |
| GSM33592 |
Denervation atrophy: expression at day 14 |
| GSM33593 |
Denervation atrophy: expression at day 14 (reverse labeling) |
|
| Relations |
| BioProject |
PRJNA90977 |
Less...
More...