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Series GSE18936 Query DataSets for GSE18936
Status Public on Jan 04, 2010
Title RNA abundance in wild-type and rtt109 -/- Candida albicans, with and without peroxide stress
Organism Candida albicans
Experiment type Expression profiling by array
Summary Candida albicans is a ubiquitous opportunistic pathogen that is the most prevalent cause of hospital-acquired fungal infections. In mammalian hosts, C. albicans is engulfed by phagocytes that attack the pathogen with DNA-damaging reactive oxygen species (ROS). Acetylation of histone H3 lysine 56 (H3K56) by the fungal-specific histone acetyltransferase Rtt109 is important for yeast model organisms to survive DNA damage and maintain genome integrity. To assess the importance of Rtt109 for C. albicans pathogenicity, we deleted the predicted homologue of Rtt109 in the clinical C. albicans isolate, SC5314. C. albicans rtt109 -/- mutant cells lack acetylated H3K56 (H3K56ac) and are hypersensitive to genotoxic agents. Additionally, rtt109 -/- mutant cells constitutively display increased H2A S129 phosphorylation and elevated DNA repair gene expression, consistent with endogenous DNA damage.
 
Overall design Four independent pairs of biological replicate samples were analyzed to compare RNA levels in wild-type and rtt109 -/- cells. Two experiments were performed: first, in unperturbed cells grown in YPD and second, in cells exposed to H2O2. To account for dye effects, two of the four samples for each experiment were analyzed using Cy3 labeling of the wild-type sample and Cy5 labeling of the mutant sample; the dyes were swapped for the other two biological replicates.
 
Contributor(s) Lopes da Rosa J, Boyartchuk VL, Zhu LJ, Kaufman PD
Citation(s) 20080646
Submission date Nov 09, 2009
Last update date Mar 21, 2012
Contact name Jessica Lopes da Rosa-Spiegler
E-mail(s) jessica.lopesdarosa@umassmed.edu
Organization name University of Massachusetts Medical School
Street address 364 Plantation street
City Worcester
State/province MA
ZIP/Postal code 01605
Country USA
 
Platforms (1)
GPL9545 The Genome Center at Washington University, Candida albicans 20K array
Samples (8)
GSM469145 YPD_rep1
GSM469146 YPD_rep2
GSM469147 YPD_rep3
Relations
BioProject PRJNA121051

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE18936_RAW.tar 21.0 Mb (http)(custom) TAR (of GPR)
Processed data included within Sample table

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