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Series GSE189387 Query DataSets for GSE189387
Status Public on Nov 27, 2023
Title SENP2 suppresses browning of white adipose tissues by de-conjugating SUMO from C/EBPβ
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The adipose tissue is a key site regulating energy metabolism. One of the contributing factors behind this is browning of white adipose tissue (WAT), however, knowledge of the intracellular determinants of browning process remains incomplete. By generating adipocyte-specific Senp2 knockout (Senp2-aKO) mice, here we showed that SENP2 negatively regulates browning by de-conjugating SUMO from C/EBPβ. Senp2-aKO mice were resistant to diet-induced obesity and insulin resistance due to increased energy expenditure and heat production. Senp2 knockout promoted beige adipocyte accumulation in inguinal WAT by upregulation of thermogenic gene expression. In addition, SENP2 knockdown promoted thermogenic adipocyte differentiation of precursor cells isolated from inguinal and epididymal WATs. Mechanistically, sumoylated C/EBPβ, a target of SENP2, suppressed expression of HOXC10, a browning inhibitor, by recruiting a transcriptional repressor DAXX. These findings indicate that a SENP2-C/EBPβ-HOXC10 axis operates for the control of beige adipogenesis in inguinal WAT.
Overall design The transcriptomic profiles of SVF-derived adipocytes of WATs (eWAT and iWAT) of control or Senp2-aKO mice with and without C/EBPβ knockdown.
Web link
Contributor(s) Lee J, Chae S, Hwang D, Chung S, Park K
Citation(s) 35196497
Submission date Nov 23, 2021
Last update date Nov 28, 2023
Contact name Sehyun Chae
Organization name Korea Brain Research Institute
Department Neurovascular Unit Research Group
Street address 61, Cheomdan-ro, Dong-gu
City Daegu
ZIP/Postal code 41062
Country South Korea
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (24)
GSM5700439 e1-1: eWAT_Cont_siNS_1
GSM5700440 e2-1: eWAT_Cont_siNS_2
GSM5700441 e3-1: eWAT_Cont_siNS_3
BioProject PRJNA782879

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Supplementary file Size Download File type/resource
GSE189387_RAW.tar 5.2 Mb (http)(custom) TAR (of TXT)
GSE189387_Raw_counts_matrix.txt.gz 909.7 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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