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Original Data from GPR file |
Original Data from GPR file |
Original Data from GPR file |
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Filter out bad data (-100) and separate background data (blank and empty) from ORF data |
Filter out bad data (-100) and separate background data (blank and empty) from ORF data |
Filter out bad data (-100) and separate background data (blank and empty) from ORF data |
Filter out bad data (-100) and separate background data (blank and empty) from ORF data |
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Determine median of background 635 and 532 and separate data into upper and lower halves |
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Determine median of background 635 and 532 and separate data into upper and lower halves |
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Determine the median of the lower half (=25th percentile) and upper half (=75th percentile) and interquartile range (3/2 the difference between the 25th and 75th percentile +/- 75th and 25th percentile) |
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Correcting background (keep background between upper and lower IQR |
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Final background (if Cbackground is blank in one eliminate in the other to be consistent) |
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"Calculate Background average from final background, standard deviation and threshold (mean +2SD)" |
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"Elimination of 635 value if below threshold, replacement of 532 value with threshold if below threshold" |
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Calculation of correction value to adjust each array. Average of normalized data points (532/635) |
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Division of correction value (AM) from each 532/635 ratio (AJ) |