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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Nov 01, 2022 |
| Title |
The bone marrow niche regulates redox and energy balance in leukemia stem cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The Cxcr4-Cxcl12 axis has been postulated as a critical pathway dictating leukemia stem cell (LSCs) chemoresistance in AML due to its role in controlling cellular egress from the marrow. Nevertheless, the cellular source of Cxcl12 in the AML microenvironment and the mechanism by which Cxcl12 exert its protective role in AML in vivo remain unresolved. We have evaluated the functional role of Cxcl12 secreted by early mesenchymal stromal cells (MSCs) and osteolineage committed cells in acute myeloid leukemia (AML) maintenance in vivo. Our results demonstrate that early MSCs, in contrast to committed osteoblasts, are integral part of the MLL::AF9 derived AML niche and control LSCs maintenance through Cxcl12 secretion. Cxcl12 from MSCs regulates the oxidative state of LSCs and promotes energy metabolism. Furthermore, the protective role of the niche through the activation of the CXCL12-CXCR4 axis, may also represent a biological hallmark in human pediatric and adult AML, hence, reinforcing the notion that targeting the MSCs-derived CXCL12 may help eradicate leukemia.
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| Overall design |
LSCs mRNA profiles from Prx1-Cxcl12-Ctrl and Prx1-Cxcl12-Mut mice (n=2 technical replicates of 9 mice and 7000 cells/mouse) 30 days post leukemia induction.
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| Contributor(s) |
Saez B, Prosper F |
| Citation(s) |
35618797 |
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| Submission date |
Dec 01, 2021 |
| Last update date |
Jan 31, 2023 |
| Contact name |
Itziar Cenzano |
| E-mail(s) |
icenzano@unav.es
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| Organization name |
CIMA Universidad de Navarra
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| Street address |
Avenida de Pio XII, 55
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| City |
Pamplona |
| State/province |
Navarra |
| ZIP/Postal code |
31008 |
| Country |
Spain |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (18)
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| GSM5710048 |
LSCs, cxcl12_MUT12_2 |
| GSM5710049 |
LSCs, cxcl12_MUT6_1 |
| GSM5710050 |
LSCs, cxcl12_MUT6_2 |
| GSM5710051 |
LSCs, cxcl12_MUT7_1 |
| GSM5710052 |
LSCs, cxcl12_MUT7_2 |
| GSM5710053 |
LSCs, cxcl12_MUT8_1 |
| GSM5710054 |
LSCs, cxcl12_MUT8_2 |
| GSM5710055 |
LSCs, cxcl12_WT1_1 |
| GSM5710056 |
LSCs, cxcl12_WT1_2 |
| GSM5710057 |
LSCs, cxcl12_WT2_1 |
| GSM5710058 |
LSCs, cxcl12_WT2_2 |
| GSM5710059 |
LSCs, cxcl12_WT3_1 |
| GSM5710060 |
LSCs, cxcl12_WT3_2 |
| GSM5710061 |
LSCs, cxcl12_WT4_1 |
| GSM5710062 |
LSCs, cxcl12_WT4_2 |
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| Relations |
| BioProject |
PRJNA785145 |
| SRA |
SRP348992 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE189928_Countsmatrix_Cxcl12.txt.gz |
882.2 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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