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| Status |
Public on Nov 28, 2023 |
| Title |
Expression profiling and function analysis identified new microRNAs regulating cumulus expansion and apoptosis in cumulus cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
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| Summary |
RNA- and miRNA-sequencing and bioinformatics using CCs from these cultured COCs/OOXs identified candidate apoptosis- and/or CE-regulating miRNAs. Transfection of COCs/OOXs with miRNA mimic or inhibitor validated that miR-212-5p and 149-5p promoted CE by facilitating Has2 expression; miR-31-5p and 27a-3p promoted CE by increasing both Has2 and Ptx3 expression; and miR-351-5p and 503-5p inhibited CE by suppressing Ptx3 expression. Furthermore, miR-212-5p, 149-5p and Nov798 inhibited CC apoptosis, involving both Bcl2/Bax and Fas signaling. Analysis using in vivo matured COCs further verified the above apoptosis- and/or CE-regulating miRNAs except for miR-149-5p. In conclusion, this study identified and validated new CE- and apoptosis-regulating miRNAs in CCs, which can be used as biomarkers to select competent oocytes/embryos and for elucidating how the oocyte-derived factors regulate CE and CC apoptosis.
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| Overall design |
we established that mouse cumulus-oocyte complexes (COCs) cultured in expansion-supporting medium supported full CE while suffering mild apoptosis, whereas mouse oocytectomized COCs (OOXs) cultured in apoptosis-triggering medium underwent severe apoptosis while supporting no CE.
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| Contributor(s) |
Tan J, Gong S, Han X, Zhang M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Dec 01, 2021 |
| Last update date |
Nov 28, 2023 |
| Contact name |
min zhang |
| E-mail(s) |
gongshuai5@sdau.edu.cn
|
| Phone |
18853886109
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| Organization name |
ShanDongAgricultural University
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| Department |
College of Animal Science and Technology
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| Lab |
Animal Embryo Engineering
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| Street address |
61 Daizong Street
|
| City |
TaiAn |
| State/province |
ShanDong |
| ZIP/Postal code |
271000 |
| Country |
China |
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| Platforms (1) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA785259 |
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