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Series GSE189959 Query DataSets for GSE189959
Status Public on Nov 28, 2023
Title Expression profiling and function analysis identified new microRNAs regulating cumulus expansion and apoptosis in cumulus cells
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
Summary RNA- and miRNA-sequencing and bioinformatics using CCs from these cultured COCs/OOXs identified candidate apoptosis- and/or CE-regulating miRNAs. Transfection of COCs/OOXs with miRNA mimic or inhibitor validated that miR-212-5p and 149-5p promoted CE by facilitating Has2 expression; miR-31-5p and 27a-3p promoted CE by increasing both Has2 and Ptx3 expression; and miR-351-5p and 503-5p inhibited CE by suppressing Ptx3 expression. Furthermore, miR-212-5p, 149-5p and Nov798 inhibited CC apoptosis, involving both Bcl2/Bax and Fas signaling. Analysis using in vivo matured COCs further verified the above apoptosis- and/or CE-regulating miRNAs except for miR-149-5p. In conclusion, this study identified and validated new CE- and apoptosis-regulating miRNAs in CCs, which can be used as biomarkers to select competent oocytes/embryos and for elucidating how the oocyte-derived factors regulate CE and CC apoptosis.
Overall design we established that mouse cumulus-oocyte complexes (COCs) cultured in expansion-supporting medium supported full CE while suffering mild apoptosis, whereas mouse oocytectomized COCs (OOXs) cultured in apoptosis-triggering medium underwent severe apoptosis while supporting no CE.
Contributor(s) Tan J, Gong S, Han X, Zhang M
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Submission date Dec 01, 2021
Last update date Nov 28, 2023
Contact name min zhang
Phone 18853886109
Organization name ShanDongAgricultural University
Department College of Animal Science and Technology
Lab Animal Embryo Engineering
Street address 61 Daizong Street
City TaiAn
State/province ShanDong
ZIP/Postal code 271000
Country China
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (12)
GSM5710573 RNA_seq _F1
GSM5710574 RNA_seq _F2
GSM5710575 RNA_seq _F3
BioProject PRJNA785259

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE189959_RAW.tar 9.3 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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