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Series GSE190049 Query DataSets for GSE190049
Status Public on Nov 01, 2023
Title RNA Polymerase II ChipSeq measurement of Pcf11 mutations in yeast
Organism Saccharomyces cerevisiae
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary High Throughput bulk-sample ChIP-seq data targeted to RNA-polymerase II was collected for duplicate samples of two distinct mutations of Pcf11 and a matched wildtype w303 samples under standard growth conditions. The overall goal was quantification of changes in transcriptional activity in response to these mutations.
Overall design High throughput DNA-sequencing data was obtained for 6 yeast samples (w303 background), with duplicate samples of two mutations of Pcf11 (YDR228C), Pcf11-13 and Pcf11-dQN, and matched wildtype. Sequence was obtained for RNApolymerase II associated samples (using the anti-pan CTD mouse monoclonal antibody) and for matched input control
Contributor(s) Graber JH, Kuehner J, Kaczmarek-Michaels K, Maki NJ, Wilson C, Pearson E, Puleo F, McGrath C, Hoskinson D, Moore CL
Citation(s) 37967370
Submission date Dec 02, 2021
Last update date Nov 16, 2023
Contact name Joel H Graber
Phone 207-288-9880
Organization name MDI Biological Laboratory
Department Bioinformatics Core
Street address 159 Old Bar Harbor Rd
City Bar Harbor
State/province ME
ZIP/Postal code 04609
Country USA
Platforms (1)
GPL17342 Illumina HiSeq 2500 (Saccharomyces cerevisiae)
Samples (12)
GSM5712616 Pcf11-DNL_1_input
GSM5712617 Pcf11-DNL_1_Pol-II
GSM5712618 Pcf11-DNL_2_input
This SubSeries is part of SuperSeries:
GSE190052 Gene expression profiling and RNA Polymerase II ChipSeq measurement of Pcf11 mutations in yeast
BioProject PRJNA785637

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE190049_RAW.tar 75.0 Mb (http)(custom) TAR (of BW)
GSE190049_pcf11_chip_input.counts.txt.gz 141.0 Kb (ftp)(http) TXT
GSE190049_pcf11_chip_pol2.counts.txt.gz 150.2 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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