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Series GSE190461 Query DataSets for GSE190461
Status Public on Dec 01, 2023
Title LncRNA RP3-340N1.2 affects the progression of lung adenocarcinoma cell lines by targeting miR-134-5p
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
Summary We found lncRNA RP3-340N1.2 is highly expressed in LUAD and relatived with the progression of LUAD through high-throughput sequencing. Our results showed that the expression level of RP3-340N1.2 was upregulated significantly in three LUAD cell lines (A549, NCI_x001E_H1299, and NCI-H1975) and lentivirus-mediated knockdown of RP3-340N1.2 inhibited the differentiation, proliferation, migration, invasion, cell cycle, and other biological processes of tumor cells significantly. Bioinformatic software predicted the downstream target of RP3-340N1.2, which was verified using dual luciferase assays and other methods. We found that RP3-340N1.2 can competitively bind miR-134-5p in LUAD, and this microRNA has a significant regulatory effect on the expression of epidermal growth factor receptor (EGFR), which leads to increased migration and invasion of LUAD cells. This discovery provided a possible mechanism by which RP3-340N1.2 participates in the development of LUAD. In conclusion, our results showed that lncRNA RP3_x001E_340N1.2 regulates the expression of EGFR indirectly by targeting miR-134-5p and promotes the progression of LUAD. Therefore, RP3-340N1.2 may become a valuable biomarker and therapeutic target in LUAD.
 
Overall design Examination of lncRNAs in lung adenocarcinoma cell lines.
 
Contributor(s) Li W, Wang C
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Submission date Dec 08, 2021
Last update date Dec 01, 2023
Contact name Wei Li
E-mail(s) w17865197265@outlook.com
Phone (+86)17865197265
Organization name Shandong University
Street address Wenhuaxi Road NO.44
City Jinan
State/province Shandong
ZIP/Postal code 250000
Country China
 
Platforms (1)
GPL20795 HiSeq X Ten (Homo sapiens)
Samples (8)
GSM5724103 T1
GSM5724104 T2
GSM5724105 T3
Relations
BioProject PRJNA787235

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE190461_lncRNA_fpkm.txt.gz 604.8 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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