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Series GSE190461 Query DataSets for GSE190461
Status Public on Dec 01, 2023
Title LncRNA RP3-340N1.2 affects the progression of lung adenocarcinoma cell lines by targeting miR-134-5p
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
Summary We found lncRNA RP3-340N1.2 is highly expressed in LUAD and relatived with the progression of LUAD through high-throughput sequencing. Our results showed that the expression level of RP3-340N1.2 was upregulated significantly in three LUAD cell lines (A549, NCI_x001E_H1299, and NCI-H1975) and lentivirus-mediated knockdown of RP3-340N1.2 inhibited the differentiation, proliferation, migration, invasion, cell cycle, and other biological processes of tumor cells significantly. Bioinformatic software predicted the downstream target of RP3-340N1.2, which was verified using dual luciferase assays and other methods. We found that RP3-340N1.2 can competitively bind miR-134-5p in LUAD, and this microRNA has a significant regulatory effect on the expression of epidermal growth factor receptor (EGFR), which leads to increased migration and invasion of LUAD cells. This discovery provided a possible mechanism by which RP3-340N1.2 participates in the development of LUAD. In conclusion, our results showed that lncRNA RP3_x001E_340N1.2 regulates the expression of EGFR indirectly by targeting miR-134-5p and promotes the progression of LUAD. Therefore, RP3-340N1.2 may become a valuable biomarker and therapeutic target in LUAD.
Overall design Examination of lncRNAs in lung adenocarcinoma cell lines.
Contributor(s) Li W, Wang C
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Submission date Dec 08, 2021
Last update date Dec 01, 2023
Contact name Wei Li
Phone (+86)17865197265
Organization name Shandong University
Street address Wenhuaxi Road NO.44
City Jinan
State/province Shandong
ZIP/Postal code 250000
Country China
Platforms (1)
GPL20795 HiSeq X Ten (Homo sapiens)
Samples (8)
GSM5724103 T1
GSM5724104 T2
GSM5724105 T3
BioProject PRJNA787235

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Supplementary file Size Download File type/resource
GSE190461_lncRNA_fpkm.txt.gz 604.8 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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