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Series GSE191105 Query DataSets for GSE191105
Status Public on Nov 16, 2022
Title NAT10-mediated N4-Acetylcytidine is Required for Spermatogenesis and Male Fertility
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary RNA modifications are rapidly emerging as critical post-transcriptional regulators which play a key role in various biological processes. N4-acetylcysteine (ac4C) is one of the “epi-transcriptome” that is present on tRNA, rRNA, mRNA and is also highly conserved in all species. However, cytidine acetylation's in vivo physiological functions remain poorly understood particularly in mammals. Here we demonstrate only known ac4C “writer”—N-acetyltransferase 10 (NAT10) plays essential roles in male reproduction. NAT10 protein and the ac4C modification have different patterns in different organs and there are dynamic changes during spermatogenesis. Germ cell-specific ablation of NAT10 severely inhibited meiotic entry and defects in synapse the homologous chromosomes, repair of DNA double-strand breaks (DSBs) during prophase of the first meiotic division. ac4C RIP-qPCR reveals genes functioning in meiotic prophase I show a decrease in Nat10fl/-; Stra8 GFP-Cre mouse testes. These findings highlight the crucial physiological functions of mRNA ac4C modification in male spermatogenesis and expand our understanding of mRNA ac4C modification in the regulation of specific physiological processes in vivo.
Overall design Examination of WT and Nat10-SKO transcriptome: A total of 17 samples were analyzed in this study, including WT and Nat10-SKO spermatogenic cells from 3 stages, spermatogonia, preleptotene and leptotene/zygotene stage.
Contributor(s) Chen L, Wang W, Wu Y, Fan H
Citation(s) 35801907
Submission date Dec 17, 2021
Last update date Nov 16, 2022
Contact name Heng-yu Fan
Organization name Life Sciences Institute
Lab Fan heng-yu
Street address Yuhangtang Road
City Hangzhou
State/province Zhejiang
ZIP/Postal code 310058
Country China
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (17)
GSM5739135 Nat10_sKO_LZ_1
GSM5739136 Nat10_sKO_LZ_2
GSM5739137 Nat10_sKO_LZ_3
BioProject PRJNA789775

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Supplementary file Size Download File type/resource
GSE191105_Nat10_male_merge_count_ERCC.txt.gz 868.4 Kb (ftp)(http) TXT
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