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Status |
Public on Feb 08, 2024 |
Title |
RNA-sequencing analysis reveals the different gene expression in male and female wild type and TECRL knockout mice. |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Purpose: In previous study, we generated the TECRL knockout mice. The purpose of this study is to indentify the implication function of TECRL on cardiac myocytes in male and female TECRL knockout(TECRL-/-) mice compared with wild type(WT) mice. Methods: Total RNA profiles of 5 males WT mice and 5 males TECRL-/- mice, 3 females WT mice and 7 females TECRL-/- mice were generated by RNA sequencing. The first strand cDNA was synthesized using random hexamer primer and the second strand cDNA synthesis was performed using DNA Polymeraseâ… and RNase H. Results: 1249 genes were identified between TECRL-/- mice and WT mice through RNA-seq analysis. GO analysis and KEGG analysis revealed some pathways, especially lipid metabolism and Ca2+ handling were closedly related to TECRL deficiency.
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Overall design |
Comparation of transcriptome of heart from 8 weeks male and female wild type(WT) and TECRL-/- mice.
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Contributor(s) |
Lin S, Hou C, Chen S, Xie L, Xiao T |
Citation(s) |
38283583 |
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Submission date |
Dec 17, 2021 |
Last update date |
Feb 08, 2024 |
Contact name |
Shujia Lin |
E-mail(s) |
linshujia96@sjtu.edu.cn
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Organization name |
Shanghai Children’s Hospital
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Street address |
No. 355 Luding Road, Shanghai, China
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City |
Shanghai |
ZIP/Postal code |
200062 |
Country |
China |
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Platforms (1) |
GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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Samples (20)
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Relations |
BioProject |
PRJNA789784 |