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Series GSE192470 Query DataSets for GSE192470
Status Public on Jan 26, 2022
Title Early anti-tumor activity of oral Langerhans cells is compromised by a carcinogen
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Early diagnosis of oral squamous cell carcinoma (OSCC) remains an unmet clinical need. Therefore, elucidating the initial events of OSCC preceding tumor development could benefit OSCC prognosis. Here we define the Langerhans cells (LC) of the tongue and demonstrate that LC protect the epithelium from carcinogen-induced OSCC by rapidly priming αβT cells capable of eliminating γH2AX+ epithelial cells, whereas γδT and NK cells are dispensable. The carcinogen, however, dysregulates the epithelial resident mononuclear phagocytes, reducing LC frequencies while dendritic cells (DC), macrophages, and plasmacytoid DC (pDC) populate the epithelium. Single-cell RNAseq analysis indicates that these newly differentiated cells display an immunosuppressive phenotype accompanied by an expansion of T regulatory (Treg) cells. Accumulation of the Treg cells was regulated, in part, by pDC and precedes the formation of visible tumors. This suggests LC play an early protective role during OSCC, yet the capacity of the carcinogen to dysregulate the differentiation of mononuclear phagocytes facilitates oral carcinogenesis.
 
Overall design The mice were treated with the carcinogen 4NQO in the drinking water to induce carcinogenesis in the tongue epithelium, the scRNAseq analysis was performed on cells purified from the tongue epithelium after enrichment to CD45+ cells.
 
Contributor(s) Hovav A, Yona S, Saba Y, David E
Citation(s) 35012988
Submission date Dec 22, 2021
Last update date Jan 27, 2022
Contact name Ido Amit
E-mail(s) ido.amit@weizmann.ac.il
Phone 972-8-9343338
Organization name Weizmann Institute of Science
Department Immunology
Street address 234 Herzl st.
City Rehovot
ZIP/Postal code 760001
Country Israel
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (2)
GSM5747606 4NQO
GSM5747607 Naïve
Relations
BioProject PRJNA791624

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Supplementary file Size Download File type/resource
GSE192470_RAW.tar 44.3 Mb (http)(custom) TAR (of TAR)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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