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Series GSE19248 Query DataSets for GSE19248
Status Public on Mar 18, 2010
Title Performance Evaluation of Commercial miRNA Expression Array Platforms
Platform organisms Homo sapiens; Mus musculus; Rattus norvegicus; Human alphaherpesvirus 1; Human betaherpesvirus 5; human gammaherpesvirus 4; JC polyomavirus; Human immunodeficiency virus 1; synthetic construct; Human gammaherpesvirus 8; Betapolyomavirus hominis; Betapolyomavirus macacae
Sample organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary This data is part of a miRNA platform comparison study. We compared the performance characteristics of four commercial miRNA array technologies and found that all platforms performed well in separate measures of performance. The Ambion and Agilent platforms were more accurate, whereas the Illumina and Exiqon platforms were more specific. Furthermore, the data analysis approach had a large impact on the performance, predominantly by improving precision.
Overall design Performance of four (4) commercially available miRNA platforms was evaluated using 7 placenta samples spiked with synthetic microRNA spikes (in Latin-square design) absent in placenta. Platforms were primarily evaluated for accuracy and specificity.
Contributor(s) Sah S, Wilson M, Eveleigh D, McCall MN, Irizarry R
Citation(s) 20298588
Submission date Dec 01, 2009
Last update date Mar 21, 2012
Contact name Sachin Sah
Organization name Asuragen Inc.
Street address 2150 Woodward St, Suite 100
City Austin
State/province TX
ZIP/Postal code 78744
Country USA
Platforms (4)
GPL7724 miRCURY LNA microRNA Array, v. 9.2, all organisms
GPL8178 Illumina Human v1 MicroRNA expression beadchip
GPL9081 Agilent-016436 Human miRNA Microarray 1.0 G4472A (miRNA ID version)
Samples (28)
GSM477719 Placenta_Spike_Pool1_Agilent
GSM477720 Placenta_Spike_Pool2_Agilent
GSM477721 Placenta_Spike_Pool3_Agilent
BioProject PRJNA120787

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE19248_Illumina_Human_miRNA_RAW.txt.gz 19.1 Kb (ftp)(http) TXT
GSE19248_RAW.tar 31.0 Mb (http)(custom) TAR (of CEL, TXT, XML)
Processed data included within Sample table

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