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Series GSE192607 Query DataSets for GSE192607
Status Public on Sep 30, 2023
Title Function of ARGONAUTE proteins in the Arabidopsis shoot apical meristem (SAM) [RIP-seq]
Organism Arabidopsis thaliana
Experiment type Other
Summary In sexually propagating organisms, genetic and epigenetic mutations are evolutionarily relevant only if they occur in the germline and provide inherited information to the next generation. In contrast to most animals, plants are thought to lack an early segregating germline, implying that somatic cells can contribute genetic information to the progeny if they differentiate into meiocytes. Here we demonstrate that two ARGONAUTE proteins, AGO5 and AGO9, are expressed in the reproductive lineage throughout development and mark an early-segregating germline. Furthermore, both AGOs are loaded with dynamic populations of small RNAs derived from highly methylated, pericentromeric, long transposons. Sequencing single nuclei revealed that many of these transposons are highly expressed within a central stem cell domain. This indicates a host-parasite tug of war and specific silencing pathways in plant shoot apical meristems (SAMs). Our results open the path to investigate transposon biology and epigenome dynamics at cellular resolution in the SAM stem cell niche.
 
Overall design Analysis of AGO5- and AGO9-bound sRNAs at 7 days and 35 days after germination
 
Contributor(s) Gutzat R, Nguyen VH, Bradamante G
Citation(s) 38060984
Submission date Dec 26, 2021
Last update date Apr 04, 2024
Contact name Ruben Gutzat
E-mail(s) ruben.gutzat@gmi.oeaw.ac.at
Phone 00431790449831
Organization name Gregor Mendel Institute GmbH
Lab Mittelsten Scheid
Street address Dr. Bohr-Gasse 3
City Vienna
State/province Vienna
ZIP/Postal code 1030
Country Austria
 
Platforms (1)
GPL21785 Illumina HiSeq 4000 (Arabidopsis thaliana)
Samples (16)
GSM5753058 RIP-seq AGO5 D7 rep1
GSM5753059 RIP-seq AGO5 D7 rep2
GSM5753060 RIP-seq AGO9 D7 rep1
This SubSeries is part of SuperSeries:
GSE192611 Function of ARGONAUTE proteins in the Arabidopsis shoot apical meristem (SAM)
Relations
BioProject PRJNA792340

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE192607_processed_RIP_seq_RPM_table.txt.gz 9.3 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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