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Series GSE192868 Query DataSets for GSE192868
Status Public on Apr 01, 2022
Title Chromosome remodelling by SMC/Condensin in B. subtilis is regulated by Soj/ParA during growth and sporulation
Organism Bacillus subtilis
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary SMC complexes, loaded at ParB-parS sites, are key mediators of chromosome organization in bacteria. ParA/Soj proteins interact with ParB/Spo0J in a pathway involving ATP-dependent dimerization and DNA binding, leading to chromosome segregation and SMC loading. In Bacillus subtilis, ParA/Soj also regulates DNA replication initiation, and along with ParB/Spo0J is involved in cell cycle changes during endospore formation. The first morphological stage in sporulation is the formation of an elongated chromosome structure called an axial filament. We now show that a major redistribution of SMC complexes drives axial filament formation, in a process regulated by ParA/Soj. Unexpectedly, this regulation is dependent on monomeric forms of ParA/Soj that cannot bind DNA or hydrolyse ATP. These results reveal a new role for ParA/Soj proteins in the regulation of SMC dynamics in bacteria, and yet further complexity in the web of interactions involving chromosome replication, segregation, and organization, controlled by ParAB and SMC.
 
Overall design ChIP-seq against ScpB of Bacillus subtilis cells during sporulation
 
Contributor(s) Anchimiuk A, Roberts D, Gruber S, Errington J
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Submission date Jan 01, 2022
Last update date Apr 01, 2022
Contact name Anna Anchimiuk
E-mail(s) stephan.gruber@unil.ch
Organization name University of Lausanne
Department Department of Fundamental Microbiology
Lab Chromosome organization and dynamics
Street address UNIL-Sorge District
City Lausanne
State/province Vaud
ZIP/Postal code 1015
Country Switzerland
 
Platforms (2)
GPL19910 Illumina HiSeq 2500 (Bacillus subtilis)
GPL29515 Illumina HiSeq 4000 (Bacillus subtilis)
Samples (21)
GSM5767759 DMR312_t0_uninduced
GSM5767760 DMR312_t0_induced
GSM5767761 DMR312_t60_uninduced
Relations
BioProject PRJNA793572

Download family Format
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Supplementary file Size Download File type/resource
GSE192868_sporulation_1kb_ChIPseq_DMR178_179_181.txt.gz 97.6 Kb (ftp)(http) TXT
GSE192868_sporulation_1kb_ChIPseq_DMR312_314_363.txt.gz 542.6 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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