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Series GSE192945 Query DataSets for GSE192945
Status Public on Mar 06, 2022
Title Biomarkers for canine Leptospira vaccine potency
Organism Canis lupus
Experiment type Expression profiling by high throughput sequencing
Summary We used RNAseq to investigate innate immune responsiveness in canine 030-D cell line induced by inactivated L. interrogans serogroups Canicola and Icterohaemorrhagiae, and two bivalent, non-adjuvanted canine Leptospira vaccines containing the same serogroups. We identified more than 900 DEGs associated with pathways related to innate immune responses in common to these three stimuli. Several molecules including CXCL-10, SAA, and complement factor C3 were identified that could serve as targets for development of a biomarker-based in vitro assay to assess Leptospira vaccine quality. In vitro assay could replace the current animal vaccine-challenge potency assay and contribute to reduction of animal use in vaccine manufacturing.
Overall design 39 samples comprising 6 stimuli (including medium-only control) and 3 time-points. Each condition was performed in triplicate.
Contributor(s) Novak A, Pennings J, Sloots A
Citation(s) 35927283
Submission date Jan 03, 2022
Last update date Aug 06, 2022
Contact name Jeroen Pennings
Phone +31 88 689 2214
Organization name Natl. Inst. Public Health & Environment
Street address A. van Leeuwenhoeklaan 9
City Bilthoven
ZIP/Postal code 3721MA
Country Netherlands
Platforms (1)
GPL31163 NextSeq 550 (Canis lupus)
Samples (39)
GSM5769384 S01
GSM5769385 S02
GSM5769386 S03
BioProject PRJNA793869

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GSE192945_Countdata.xlsx 5.5 Mb (ftp)(http) XLSX
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