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Series GSE193448 Query DataSets for GSE193448
Status Public on Jan 13, 2022
Title Real-time quantitative PCR analysis of human dental pulp stem cells
Organism Homo sapiens
Experiment type Expression profiling by RT-PCR
Summary Excised pulp tissue was digested in a solution containing 3 mg/ml collagenase type I (Merck KGaA, Darmstadt, Germany) and 4mg/ml dispase (Merck KGaA, Darmstadt, Germany) for 1 h at 37°C. After digestion, 5 volumes of α-MEM medium containing 10% FCS were added. This solution was centrifuged at 120 × g for 10min, at room temperature. The precipitated material was resuspended in α-MEM medium and filtered through filters with pores of 70 μM. This procedure resulted in a single-cell suspension for in vitro culture. Isolated DPSCs were randomly assigned to the following experimental groups: Group 1–LPS stimulus and laser irradiation; Group 2–LPS stimulus and no laser irradiation; Group 3–Control group, no LPS or laser treatment.
 
Overall design The isolated dental pulp stem cells were stimulated to an inflammatory state, using a lipopolysaccharide (LPS) model, and then subjected or not to laser photobiomodulation. Each experiment was statistically evaluated according to the sample distribution. A total of 85 genes related to inflammation and osteogenesis were evaluated regarding their expression by RT-PCR.
 
Contributor(s) Tersariol IL, Carrilho MO
Citation(s) 35111053
Submission date Jan 11, 2022
Last update date Feb 08, 2022
Contact name Fabio Dupart Nascimento
E-mail(s) fdnascimento@gmail.com
Phone 11985584947
Organization name Federal University of Sao Paulo
Department Biochemistry
Street address Rua Doutor Gabriel Piza, 636 apto 23
City São Paulo
State/province SP
ZIP/Postal code 02036-011
Country Brazil
 
Platforms (1)
GPL31218 RT² Profiler™ PCR Array Human Osteogenesis
Samples (6)
GSM5813586 Control replicate 1
GSM5813587 Control replicate 2
GSM5813588 Lipopolysaccharides 24h induced DPSCs replicate 1
Relations
BioProject PRJNA796528

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE193448_GA_RT-PCR.xls.gz 35.3 Kb (ftp)(http) XLS
GSE193448_LPS.pdf 431.9 Kb (ftp)(http) PDF
Processed data are available on Series record

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