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Series GSE193448 Query DataSets for GSE193448
Status Public on Jan 13, 2022
Title Real-time quantitative PCR analysis of human dental pulp stem cells
Organism Homo sapiens
Experiment type Expression profiling by RT-PCR
Summary Excised pulp tissue was digested in a solution containing 3 mg/ml collagenase type I (Merck KGaA, Darmstadt, Germany) and 4mg/ml dispase (Merck KGaA, Darmstadt, Germany) for 1 h at 37°C. After digestion, 5 volumes of α-MEM medium containing 10% FCS were added. This solution was centrifuged at 120 × g for 10min, at room temperature. The precipitated material was resuspended in α-MEM medium and filtered through filters with pores of 70 μM. This procedure resulted in a single-cell suspension for in vitro culture. Isolated DPSCs were randomly assigned to the following experimental groups: Group 1–LPS stimulus and laser irradiation; Group 2–LPS stimulus and no laser irradiation; Group 3–Control group, no LPS or laser treatment.
Overall design The isolated dental pulp stem cells were stimulated to an inflammatory state, using a lipopolysaccharide (LPS) model, and then subjected or not to laser photobiomodulation. Each experiment was statistically evaluated according to the sample distribution. A total of 85 genes related to inflammation and osteogenesis were evaluated regarding their expression by RT-PCR.
Contributor(s) Tersariol IL, Carrilho MO
Citation(s) 35111053
Submission date Jan 11, 2022
Last update date Feb 08, 2022
Contact name Fabio Dupart Nascimento
Phone 11985584947
Organization name Federal University of Sao Paulo
Department Biochemistry
Street address Rua Doutor Gabriel Piza, 636 apto 23
City São Paulo
State/province SP
ZIP/Postal code 02036-011
Country Brazil
Platforms (1)
GPL31218 RT² Profiler™ PCR Array Human Osteogenesis
Samples (6)
GSM5813586 Control replicate 1
GSM5813587 Control replicate 2
GSM5813588 Lipopolysaccharides 24h induced DPSCs replicate 1
BioProject PRJNA796528

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE193448_GA_RT-PCR.xls.gz 35.3 Kb (ftp)(http) XLS
GSE193448_LPS.pdf 431.9 Kb (ftp)(http) PDF
Processed data are available on Series record

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