|
Status |
Public on Jul 17, 2023 |
Title |
Early rotarod training aggravates injury and impairs functional recovery after spinal cord injury |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
|
Summary |
Mice with rotarod training showed significant differences in the expression of trophic factors and cytokines, histological repair, and functional recovery when compared with the mice without training. To exlore the potential mechanism, we applied the next-generation sequence to compare the transcriptome profile between two groups. We found that there were 1299 DEGs between two groups with 586 upregulated genes and 713 downregulated genes. GO enrichment analysis revealed that DEGs, especially the downregulated genes, were primarily associated with revascularization biological process like angiogenesis, regulation of vasculature development, regulation of angiogenesis, and cellular component including postsynapse, neuron to neuron synapse, etc. On the other hand, KEGG pathway analysis showed that those significantly altered genes, also downregulated DEGs in particular, were mainly enriched in the PI3K-Akt signaling pathway.
|
|
|
Overall design |
Explore the gene expression profile between mice with or without rotarod training after SCI. Mice were randomly assigned to two groups: SCIT (mice with rotarod training) and SCI (mice without rotarod training).
|
|
|
Contributor(s) |
Junhao D, Fanqi M, Licheng Z, Peifu T |
Citation |
https://doi.org/10.1016/j.heliyon.2023.e18158
|
|
Submission date |
Jan 17, 2022 |
Last update date |
Jul 17, 2023 |
Contact name |
Junhao Deng |
E-mail(s) |
deng_junh@yeah.net
|
Phone |
15811173069
|
Organization name |
Chinese PLA General Hospital
|
Street address |
Fuxing Road, Beijing, 100853, People’s Republic of China.
|
City |
Beijing |
State/province |
Beijing |
ZIP/Postal code |
100853 |
Country |
China |
|
|
Platforms (1) |
GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
Samples (6)
|
|
Relations |
BioProject |
PRJNA798021 |