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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Nov 21, 2022 |
| Title |
Real-time quantitative PCR analysis of mouse lungs after exposure to early SARS-CoV-2 bearing 614G, Kappa or Delta variant |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by RT-PCR
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| Summary |
Age-matched K18-hACE2 transgenic mice were passively transferred with human COVID-19 mRNA post-vaccination (post-vac) sera followed by the lethal challenge with different SARS-CoV-2 viruses via intranasal route, including (1) New York-PV09158/2020 (NY) of lineage B.1.3 bearing 614G, (2) Kappa (B.1.617.1) and (3) Delta (B.1.617.2). Mouse lungs were harvested on 5 days post infection (dpi). Total RNA was extracted using QIAgen RNeasy Plus Mini Kit and was reverse transcribed using the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific). Resultant cDNA was used as the template along with RT2 SYBR Green ROX qPCR Mastermix (Qiagen) to perform RT² Profiler™ PCR Array Mouse Hypoxia Signaling Pathway (Qiagen) real-time PCR in Stratagene MX3000p qPCR system.
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| Overall design |
qPCR gene expression profiling. Lung homogenates from 6 SARS-CoV-2 infected K18-hACE2 transgenic mice on dpi 5 were treated as indicated in the summary. Total RNA extracted was analyzed individually.
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| Contributor(s) |
Xie H, Tang W |
| Citation(s) |
36373096 |
| Submission date |
Jan 17, 2022 |
| Last update date |
Nov 21, 2022 |
| Contact name |
Hang Xie |
| E-mail(s) |
Hang.Xie@fda.hhs.gov
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| Organization name |
US Food and Drug Administration, Center for Biologics Evaluation and Research
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| Street address |
10993 New Hampshire Ave WO 52/72 Rm 1324
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| City |
Silver Spring |
| State/province |
Maryland |
| ZIP/Postal code |
20993 |
| Country |
USA |
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| Platforms (1) |
| GPL30599 |
QIAgen RT² Profiler PCR Array Mouse Hypoxia Signaling Pathway |
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| Samples (39)
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| GSM5822508 |
Lung, NY_Mock, rep1 |
| GSM5822509 |
Lung, NY_Mock, rep2 |
| GSM5822510 |
Lung, NY_Mock, rep3 |
| GSM5822511 |
Lung, NY_Mock, rep4 |
| GSM5822512 |
Lung, NY_Mock, rep5 |
| GSM5822513 |
Lung, NY_Mock, rep6 |
| GSM5822514 |
Lung, Kappa_Mock, rep1 |
| GSM5822515 |
Lung, Kappa_Mock, rep2 |
| GSM5822516 |
Lung, Kappa_Mock, rep3 |
| GSM5822517 |
Lung, Kappa_Mock, rep4 |
| GSM5822518 |
Lung, Kappa_Mock, rep5 |
| GSM5822519 |
Lung, Kappa_Mock, rep6 |
| GSM5822520 |
Lung, Delta_Mock, rep1 |
| GSM5822521 |
Lung, Delta_Mock, rep2 |
| GSM5822522 |
Lung, Delta_Mock, rep3 |
| GSM5822523 |
Lung, Delta_Mock, rep4 |
| GSM5822524 |
Lung, Delta_Mock, rep5 |
| GSM5822525 |
Lung, Delta_Mock, rep6 |
| GSM5822526 |
Lung, NY_Post-vac, rep1 |
| GSM5822527 |
Lung, NY_Post-vac, rep2 |
| GSM5822528 |
Lung, NY_Post-vac, rep3 |
| GSM5822529 |
Lung, NY_Post-vac, rep4 |
| GSM5822530 |
Lung, NY_Post-vac, rep5 |
| GSM5822531 |
Lung, NY_Post-vac, rep6 |
| GSM5822532 |
Lung, Kappa_Post-vac, rep1 |
| GSM5822533 |
Lung, Kappa_Post-vac, rep2 |
| GSM5822534 |
Lung, Kappa_Post-vac, rep3 |
| GSM5822535 |
Lung, Kappa_Post-vac, rep4 |
| GSM5822536 |
Lung, Kappa_Post-vac, rep5 |
| GSM5822537 |
Lung, Kappa_Post-vac, rep6 |
| GSM5822538 |
Lung, Delta_Post-vac, rep1 |
| GSM5822539 |
Lung, Delta_Post-vac, rep2 |
| GSM5822540 |
Lung, Delta_Post-vac, rep3 |
| GSM5822541 |
Lung, Delta_Post-vac, rep4 |
| GSM5822542 |
Lung, Delta_Post-vac, rep5 |
| GSM5822543 |
Lung, Delta_Post-vac, rep6 |
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| Relations |
| BioProject |
PRJNA798065 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE193839_fold-change.txt.gz |
4.8 Kb |
(ftp)(http) |
TXT |
| GSE193839_matrix.txt.gz |
11.3 Kb |
(ftp)(http) |
TXT |
| GSE193839_non-normalized_data.txt.gz |
7.4 Kb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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