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Series GSE194127 Query DataSets for GSE194127
Status Public on Jan 24, 2022
Title 16S rRNA gene sequence data revealed effects of natural phenylethanoids on gut microbiota.
Organism Mus musculus
Experiment type Other
Summary The aim of study is to evaluate whether salidroside (S), tyrosol (T) and hydroxytyrosol (H) which are dietary phenylethanoids of natural origins have an influence on reversing gut dysbiosis induced by metabolic syndrome (MetS) mice. C57 BL/6J female mice induced by high fructose diet were established. All mice were adapted to the environment for 7 days with normal diet and sterile drinking water (DW), and randomly divided into 6 groups. Mice in the ND group are fed with ND and treated with normal saline. Other groups were fed with high fructose (HFru) by administration of normal saline, salidroside (S), tyrosol (T) or hydroxytyrosol (H) for 12 weeks by intragastric gavage. Fresh feces from each mouse were collected one days before the end of the experiment and temporarily placed in sterile tubules, and then snap-frozen in liquid nitrogen. Total DNA from stool bacteria was extracted using QIAamp DNA stool mini kit from Qiagen (Germantown, MD, USA) according to the manufacturer’s instructions. Illumina HiSeq sequencing analysis of the DNA samples.16S rRNA gene sequence data further revealed that S, T and H could enhance the diversity of gut microbiota. In general, the abundance of Shigella, Acinetobacter, Lactobacillus, Staphylococcus and Sporosarcina had changed significantly. These findings suggest that S, T and H probably suppress lipid accumulation and to hepatoprotective effect and improve intestinal microflora disorders to attenuate metabolic syndromes.
Overall design The 16S rRNA gene sequences in stool bacteria of mice were detected in 5 different groups, with 6 samples in each group, namely control, model, salidroside (S), tyrosol (T) and hydroxytyrosol (H) group.
Contributor(s) Jiang M, Zhan X, He M
Citation(s) 35928270
Submission date Jan 21, 2022
Last update date Aug 16, 2022
Contact name miaomiao Jiang
Organization name Tianjin University of Traditional Chinese Medicine
Street address 10 Poyanghu Road, West Area, Tuanbo New Town, Jinghai District, Tianjin
City Tianjin
ZIP/Postal code 301617
Country China
Platforms (1)
GPL16417 Illumina MiSeq (Mus musculus)
Samples (30)
GSM5828867 C1
GSM5828868 C2
GSM5828869 C3
BioProject PRJNA799266

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Supplementary file Size Download File type/resource
GSE194127_seqs_process_stat.txt.gz 781 b (ftp)(http) TXT
GSE194127_summary.txt.gz 384 b (ftp)(http) TXT
GSE194127_table_f_even_depth_w_tax.txt.gz 378.7 Kb (ftp)(http) TXT
GSE194127_table_filtered_w_tax.txt.gz 418.6 Kb (ftp)(http) TXT
GSE194127_table_w_tax.txt.gz 789.6 Kb (ftp)(http) TXT
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Processed data are available on Series record

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