|
| Status |
Public on Apr 16, 2024 |
| Title |
Reliance of neuronal gene expression on cohesin scales with chromatin loop length (5C) |
| Organism |
Mus musculus |
| Experiment type |
Other
|
| Summary |
5C-seq from Rad21lox/lox;NexCre/+ and Rad21+/+;NexCre/+ dissociated cortical neurons at Day In Vitro (DIV10) without stimulation or after treatment with TTX/D-AP5 and 1h of KCl depolarization. And, 5C of and doxycycline treated Rad21-TEV primary neurons transduced with NLS-TEV
|
| |
|
| Overall design |
5C-seq from Rad21lox/lox;NexCre/+ and Rad21+/+;NexCre/+ dissociated cortical neurons at Day In Vitro (DIV10) without stimulation or after treatment with TTX/D-AP5 and 1h of KCl depolarization. And, Total of 8 samples analysed; 2 replicates of Rad21-tev primary neurons 24h following doxycyline treatment (6 hours, 100ng/ml), 2 replicates of Rad21-tev primary neurons 24h following vehicle treatment. 2 replicates of Rad21-tev primary neurons 7 days following doxycycline treatment (6 hours, 100ng/ml), 2 replicates of Rad21-tev primary neurons 7 days following vehicle treatment.
|
| |
|
| Contributor(s) |
Merkenschlager M, Beagan J, Weiss F, Wang Y |
| Citation(s) |
35471149 |
| |
| Submission date |
Jan 21, 2022 |
| Last update date |
Apr 17, 2024 |
| Contact name |
LMS Bioinformatics Core |
| E-mail(s) |
bioinformatics@lms.mrc.ac.uk
|
| Organization name |
MRC London Institute of Medical Sciences
|
| Department |
Bioinformatics Core
|
| Street address |
Hammersmith Hospital Campus, Du Cane Road
|
| City |
London |
| ZIP/Postal code |
W12 0NN |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (16)
|
| GSM5830463 |
Rad21tev+DOX24h_primary_neuron_culture Rep1 Synaptic 5C |
| GSM5830464 |
Rad21tev+DOX7D_primary_neuron_culture Rep1 Synaptic 5C |
| GSM5830465 |
Rad21tev+VEH7D_primary_neuron_culture Rep1 Synaptic 5C |
| GSM5830466 |
Rad21tev+VEH24h_primary_neuron_culture Rep1 Synaptic 5C |
| GSM5830467 |
Rad21tev+DOX24h_primary_neuron_culture Rep2 Synaptic 5C |
| GSM5830468 |
Rad21tev+DOX7D_primary_neuron_culture Rep2 Synaptic 5C |
| GSM5830469 |
Rad21tev+VEH7D_primary_neuron_culture Rep2 Synaptic 5C |
| GSM5830470 |
Rad21tev+VEH24h_primary_neuron_culture Rep2 Synaptic 5C |
| GSM5830471 |
Rad21WT primary dissociated cortical neuron treated with TTX/D-AP5 and 1h KCl Rep1 5C |
| GSM5830472 |
Rad21WT primary dissociated cortical neuron treated with TTX/D-AP5 and 1h KCl Rep2 5C |
| GSM5830473 |
Rad21WT primary dissociated cortical neuron treated with TTX/D-AP5 Rep1 5C |
| GSM5830474 |
Rad21WT primary dissociated cortical neuron treated with TTX/D-AP5 Rep2 5C |
| GSM5830475 |
Rad21KO primary dissociated cortical neuron treated with TTX/D-AP5 Rep1 5C |
| GSM5830476 |
Rad21KO primary dissociated cortical neuron treated with TTX/D-AP5 Rep2 5C |
| GSM5830477 |
Rad21KO primary dissociated cortical neuron treated with TTX/D-AP5 and 1h KCl Rep1 5C |
| GSM5830478 |
Rad21KO primary dissociated cortical neuron treated with TTX/D-AP5 and 1h KCl Rep2 5C |
|
| This SubSeries is part of SuperSeries: |
| GSE172429 |
Activity-induced gene expression and long-range enhancer-promoter contacts in cohesin-deficient neurons |
|
| Relations |
| BioProject |
PRJNA799394 |
Less...
More...