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| Status |
Public on Mar 20, 2023 |
| Title |
Quantitative analysis of tet inducible SOX18 during hematopoietic differentiation and Natural killer cell differentiation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Using inducible SOX18 human PSCs, we found overexpression of SOX18 showed change of transcriptional program at D8 especially T cell receptor and Toll like receptor signaling pathways. So this suggests SOX18 has an effect on lymphoid differentiation program. Specifically overexpressed SOX18 enhance NK cell production, and related to metabolism pathway. This finding that SOX18 promotes development of progenitors with superior NK cell potenital will advance our understanding of molecualr network regulating specification on lymphoid cells and can help to improve the scalability of NK CELL production form hPSCs form immunotherapies.
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| Overall design |
mRNA profiling of 18 samples for 5 different populations obtained from hematopoietic differentiation cultures of hESCs with doxycyclin (DOX)-inducible SOX18 expression. Samples obtained form HE isolated on day 4 (D4) of diiferentiation following D2 through D4 DOX treatment, hematopoiteic progenitors isolated on D8 of differntiation following D2-8 DOX treatment, and NK cell generated after 3 weeks of cultures of hematopoietic progenitors following DOX2-8 treatment. Cultures without DOX treatment were used as a control.
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| Contributor(s) |
Jung HS, Liu P, McIlwain S, Slukvin II |
| Citation(s) |
37250328 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 HL142665 |
Molecular Determinants of Hemogenic Endothelium |
UNIVERSITY OF WISCONSIN MADISON |
Igor I. Slukvin |
| U01 HL134655 |
Transplantation of MHC Homozygous Vascular Progenitors in Primates |
MORGRIDGE INSTITUTE FOR RESEARCH |
Igor I. Slukvin |
| P51 OD011106 |
WNPRC Supplemental Request for Nonhuman Primate Enclosures to Equip HIV/AIDS-Related Research Facilities |
UNIVERSITY OF WISCONSIN MADISON |
Steven A. Ackerman |
| P30 CA014520 |
Biomarkers for Detection and Prediction of Chemotherapy-Related Cognitive Impairment |
UNIVERSITY OF WISCONSIN MADISON |
HOWARD H. BAILEY |
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| Submission date |
Jan 28, 2022 |
| Last update date |
Jun 07, 2023 |
| Contact name |
Igor I. Slukvin |
| Organization name |
University of Wisconsin
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| Street address |
1220 Capitol Court
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| City |
Madison |
| ZIP/Postal code |
53715 |
| Country |
USA |
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| Platforms (1) |
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| Samples (18)
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| GSM5844933 |
D4, iSOX18, Dox, CD144plusKDRplusDLL4negCXCR4neg, rep1 |
| GSM5844934 |
D4, iSOX18, Dox, CD144plusKDRplusDLL4negCXCR4neg, rep2 |
| GSM5844935 |
D4, iSOX18, Dox, CD144plusKDRplusDLL4negCXCR4plus, rep1 |
| GSM5844936 |
D4, iSOX18, Dox, CD144plusKDRplusDLL4negCXCR4plus, rep2 |
| GSM5844937 |
D4, iSOX18, No DOX, CD144plusKDRplusDLL4negCXCR4neg, rep1 |
| GSM5844938 |
D4, iSOX18, No DOX, CD144plusKDRplusDLL4negCXCR4neg, rep2 |
| GSM5844939 |
D8, iSOX18, DOX, CD43plusCD235anegCD41anegCD45neg, rep1 |
| GSM5844940 |
D8, iSOX18, DOX, CD43plusCD235anegCD41anegCD45neg, rep2 |
| GSM5844941 |
D8, iSOX18, DOX, CD43plusCD235aplusCD41aplusCD45neg, rep1 |
| GSM5844942 |
D8, iSOX18, DOX, CD43plusCD235aplusCD41aplusCD45neg, rep2 |
| GSM5844943 |
D8, iSOX18, No DOX, CD43plusCD235anegCD41anegCD45neg, rep1 |
| GSM5844944 |
D8, iSOX18, No DOX, CD43plusCD235anegCD41anegCD45neg, rep2 |
| GSM5844945 |
D8, iSOX18, No DOX, CD43plusCD235aplusCD41aplusCD45neg, rep1 |
| GSM5844946 |
D8, iSOX18, No DOX, CD43plusCD235aplusCD41aplusCD45neg, rep2 |
| GSM5844947 |
D8plus3W, iSOX18, DOX, CD56plus, rep1 |
| GSM5844948 |
D8plus3W, iSOX18, DOX, CD56plus, rep2 |
| GSM5844949 |
D8plus3W, iSOX18, No DOX, CD56plus, rep1 |
| GSM5844950 |
D8plus3W, iSOX18, No DOX, CD56plus, rep2 |
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| Relations |
| BioProject |
PRJNA801680 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE195670_RAW.tar |
20.3 Mb |
(http)(custom) |
TAR (of TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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