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Series GSE195760 Query DataSets for GSE195760
Status Public on Jul 10, 2023
Title Unreprogrammed H3K9me3 prevents minor zygotic genome activation and lineage commitment in SCNT embryos
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Somatic cell nuclear transfer (SCNT) can be used to reprogram differentiated somatic cells to a totipotent state but has poor efficiency in supporting full-term development. H3K9me3 is considered to be an epigenetic barrier to zygotic genomic activation in 2-cell SCNT embryos. However, the mechanism underlying the failure of H3K9me3 reprogramming during SCNT embryo development remains elusive. Here, we perform genome-wide profiling of H3K9me3 in cumulus cell-derived SCNT embryos. We find redundant H3K9me3 marks are closely related to defective minor zygotic genome activation. Moreover, SCNT blastocysts show severely indistinct lineage-specific H3K9me3 deposition. We identify MAX and MCRS1 as potential H3K9me3-related transcription factors and are essential for early embryogenesis. Overexpression of Max and Mcrs1 significantly benefits SCNT embryo development. Notably, MCRS1 partially rescues lineage-specific H3K9me3 allocation, and further improves the efficiency of full-term development. Importantly, our data confirm the conservation of deficient H3K9me3 differentiation in Sertoli cell-derived SCNT embryos, which may be regulated by alternative mechanisms.
Overall design Smart-seq in somatic cell nuclear transfer (NT) embryos and morpholino knock down Max and Mcrs1 in fertilized blastocyst embryos.
Contributor(s) Xv R, Zhu Q
Citation(s) 37558707
Submission date Jan 31, 2022
Last update date Oct 09, 2023
Contact name Qianshu Zhu
Organization name Tongji University
Department School of Life Science and Technology
Street address Siping Road
City Shanghai
State/province Shanghai
ZIP/Postal code 270000
Country China
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (68)
GSM5849700 cumulus cell-CC_RNA_rep1
GSM5849701 cumulus cell-CC_RNA_rep2
GSM5849702 cumulus cell-CC_RNA_rep3
This SubSeries is part of SuperSeries:
GSE195762 Unreprogrammed H3K9me3 prevents minor zygotic genome activation and lineage commitment in SCNT embryos
BioProject PRJNA802118

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Supplementary file Size Download File type/resource
GSE195760_RAW.tar 1.1 Gb (http)(custom) TAR (of BW, EXP)
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Raw data are available in SRA
Processed data provided as supplementary file

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