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| Status |
Public on Jul 10, 2023 |
| Title |
Unreprogrammed H3K9me3 prevents minor zygotic genome activation and lineage commitment in SCNT embryos |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Somatic cell nuclear transfer (SCNT) can be used to reprogram differentiated somatic cells to a totipotent state but has poor efficiency in supporting full-term development. H3K9me3 is considered to be an epigenetic barrier to zygotic genomic activation in 2-cell SCNT embryos. However, the mechanism underlying the failure of H3K9me3 reprogramming during SCNT embryo development remains elusive. Here, we perform genome-wide profiling of H3K9me3 in cumulus cell-derived SCNT embryos. We find redundant H3K9me3 marks are closely related to defective minor zygotic genome activation. Moreover, SCNT blastocysts show severely indistinct lineage-specific H3K9me3 deposition. We identify MAX and MCRS1 as potential H3K9me3-related transcription factors and are essential for early embryogenesis. Overexpression of Max and Mcrs1 significantly benefits SCNT embryo development. Notably, MCRS1 partially rescues lineage-specific H3K9me3 allocation, and further improves the efficiency of full-term development. Importantly, our data confirm the conservation of deficient H3K9me3 differentiation in Sertoli cell-derived SCNT embryos, which may be regulated by alternative mechanisms.
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| Overall design |
The specific pathogen-free grade mice (SPF) grade mice, including
C57BL/6j,DBA/2 and B6D2F1 mice,were housed in the animal facility at
Tongji University, Shanghai, China. Mice were housed in individually
ventilated cages with an environment with temperature ranging from
23 to 27°C, humidity of 30–45%, and a light cycle with 12 h of light and
12 h of darkness. All the mice had free access to food and water. All
experiments were performed in accordance with the University of
Health Guide for the Care and Use of Laboratory Animals and were
approved by the Biological Research Ethics Committee of Tongji
University (TJAB04021104).
The B6D2F1 hybrid mice (8–10 weeks old) were obtained from
mating female C57BL/6j mice with male DBA/2 mice.
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| Citation(s) |
37558707 |
| |
| Submission date |
Jan 31, 2022 |
| Last update date |
Oct 09, 2023 |
| Contact name |
Qianshu Zhu |
| E-mail(s) |
zhuqianshu@tongji.edu.cn
|
| Organization name |
Tongji University
|
| Department |
School of Life Science and Technology
|
| Street address |
Siping Road
|
| City |
Shanghai |
| State/province |
Shanghai |
| ZIP/Postal code |
270000 |
| Country |
China |
| |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (146)
|
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| This SuperSeries is composed of the following SubSeries: |
| GSE194327 |
Unreprogrammed H3K9me3 prevents minor zygotic genome activation and lineage commitment in SCNT embryos |
| GSE194341 |
Unreprogrammed H3K9me3 prevents minor zygotic genome activation and lineage commitment in SCNT embryos |
| GSE195760 |
Unreprogrammed H3K9me3 prevents minor zygotic genome activation and lineage commitment in SCNT embryos |
|
| Relations |
| BioProject |
PRJNA802113 |
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