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| Status |
Public on Feb 02, 2022 |
| Title |
Expression data from OSM-overexpressing and control MDA-MB-231 triple negative breast cancer cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The cytokine Oncostatin M (OSM) promotes cancer progression by acting as central node for multicellular interactions between cancer cells and surrounding stromal cells. OSM is mainly secreted by myeloid cells and the oncostatin M receptor (OSMR) is expressed by cancer cells and cancer associated fibroblasts (CAFs), among others.
To understand the effect of OSM in triple negative breast cancer cells, a small and well-annotated Clariom S gene microarray was performed in OSM-overexpressing (MDA-MB-231-hOSM) and control (MDA-MB-231-hC) MDA-MB-231 cells.
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| Overall design |
MDA-MB-231 breast cancer cells were stably transfected with pUNO1-empty vector (MDA-MB-231-hC) and pUNO1-hOSM vector (MDA-MB-231-hOSM) and selected by adding blasticidin to the culture medium. MDA-MB-231-hC and MDA-MB-231-hOSM cells were cultured in parallel under blasticidin selection and RNA was extracted from three non-consecutive cell passages. RNA quality evaluation and microarray chips processing were performed at the Genomics Facility of the Biodonostia Health Research Institute.
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| Web link |
https://pubmed.ncbi.nlm.nih.gov/35192545/
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| Contributor(s) |
Caffarel MM, Araujo A, Azcoaga P |
| Citation(s) |
35192545 |
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| Submission date |
Jan 31, 2022 |
| Last update date |
Jun 16, 2022 |
| Contact name |
Andrea Abaurrea |
| E-mail(s) |
andrea.abaurrea@biodonostia.org
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| Organization name |
Biodonostia HRI
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| Department |
Oncology
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| Lab |
Breast Cancer
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| Street address |
Paseo Doctor Begiristain, s/n, 20014 San Sebastián
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| City |
San Sebastian |
| State/province |
Gipuzkoa |
| ZIP/Postal code |
20014 |
| Country |
Spain |
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| Platforms (1) |
| GPL23159 |
[Clariom_S_Human] Affymetrix Clariom S Assay, Human (Includes Pico Assay) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE195787 |
Effect of Oncostatin M (OSM) in breast cancer cells and cancer associated fibroblasts |
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| Relations |
| BioProject |
PRJNA802141 |
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