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Series GSE196477 Query DataSets for GSE196477
Status Public on Jan 30, 2023
Title Mutant NPM1 binds chromatin and directly regulates oncogenic transcription in acute myeloid leukemia [RNA-seq]
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary We use an endogenously degrader tagged NPM1c leukemia cell line that allows rapid small molecule induced degradation to show that NPM1c occupies specific chromatin targets in AML.
 
Overall design To investigate the transcriptional changes after loss of NPM1c, we performed RNAseq using a previously described NPM1 mutant leukemia cell line (OCI-AML3-NPM1c-FKBP12) in which only the mutant allele of NPM1 is modified to produce an endogenously tagged version of NPM1c that has an FKBP12 tag
 
Contributor(s) Uckelmann H, Hatton C
Citation(s) 36455613
Submission date Feb 10, 2022
Last update date Jan 30, 2023
Contact name Charlie Hatton
E-mail(s) Charles_Hatton@dfci.harvard.edu
Organization name Dana-Farber Cancer Institute
Department Pediatric Oncology
Lab Scott Armstrong/CPCT
Street address 450 Brookline Ave
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (24)
GSM5884968 293T-trns-AS1-HA_RNA_steadystate_REP1_211122_CGGAAT_S4
GSM5884969 293T-trns-AS1-HA_RNA_steadystate_REP2_211122_TAATCG_S8
GSM5884970 293T-trns-AS1-HA_RNA_steadystate_REP3_211122_TCGGCA_S12
This SubSeries is part of SuperSeries:
GSE196480 Mutant NPM1 binds chromatin and directly regulates oncogenic transcription in acute myeloid leukemia
Relations
BioProject PRJNA805172

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Supplementary file Size Download File type/resource
GSE196477_293T_RNAseq_DESeq2_differential_expression_summary.xlsx 29.9 Mb (ftp)(http) XLSX
GSE196477_OCIAML3_RNAseq_dTAG_24h_differentially_expressed_genes.xlsx 79.3 Kb (ftp)(http) XLSX
GSE196477_RNAseq-diffexp_OCIAML3-NPM1c-FKBP12_dTag13_OCIAML3_Selinexor_6h_Supp_S5.xlsx 417.1 Kb (ftp)(http) XLSX
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Processed data are available on Series record
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