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| Status |
Public on Jan 30, 2023 |
| Title |
Mutant NPM1 binds chromatin and directly regulates oncogenic transcription in acute myeloid leukemia [PRO-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Other
|
| Summary |
We use an endogenously degrader tagged NPM1c leukemia cell line that allows rapid small molecule induced degradation to show that NPM1c occupies specific chromatin targets in AML.
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| Overall design |
To investigate the changes in transcriptional activity and potential defects in initiation versus elongation of RNA polymerase II, we performed precisiojn run on sequenign (PRO-seq) in NPM1 mutant leukemia cell line (OCI-AML3-NPM1c-FKBP12) in which only the mutant allele of NPM1 is modified to produce an endogenously tagged version of NPM1c that has an FKBP12 tag. Treatment with dTAG-13 for 30 or 60 minutes was used to determine transcriptional changes after NPM1c degradation.
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| Contributor(s) |
Uckelmann H, Hatton C |
| Citation(s) |
36455613 |
| Submission date |
Feb 10, 2022 |
| Last update date |
Jan 30, 2023 |
| Contact name |
Charlie Hatton |
| E-mail(s) |
Charles_Hatton@dfci.harvard.edu
|
| Organization name |
Dana-Farber Cancer Institute
|
| Department |
Pediatric Oncology
|
| Lab |
Scott Armstrong/CPCT
|
| Street address |
450 Brookline Ave
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (10)
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| GSM5884986 |
OCIAML3_NPM1c-FKBP_PRO_DMSO_0m_REP1_211124_TAGCTT_S1 |
| GSM5884987 |
OCIAML3_NPM1c-FKBP_PRO_DMSO_0m_REP2_211124_GGCTAC_S2 |
| GSM5884988 |
OCIAML3_NPM1c-FKBP_PRO_dTAG13_30m_REP1_211124_CTTGTA_S3 |
| GSM5884989 |
OCIAML3_NPM1c-FKBP_PRO_dTAG13_30m_REP2_211124_AGTCAA_S4 |
| GSM5884990 |
OCIAML3_NPM1c-FKBP_PRO_dTAG13_60m_REP1_211124_AGTTCC_S5 |
| GSM5884991 |
OCIAML3_NPM1c-FKBP_PRO_dTAG13_60m_REP2_211124_ATGTCA_S6 |
| GSM5884992 |
OCIAML3_PRO_DMSO_0m_REP1_211124_CCGTCC_S7 |
| GSM5884993 |
OCIAML3_PRO_DMSO_0m_REP2_211124_GTAGAG_S8 |
| GSM5884994 |
OCIAML3_PRO_VTP_48h_REP1_211124_GTCCGC_S9 |
| GSM5884995 |
OCIAML3_PRO_VTP_48h_REP2_211124_GTGAAA_S10 |
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| This SubSeries is part of SuperSeries: |
| GSE196480 |
Mutant NPM1 binds chromatin and directly regulates oncogenic transcription in acute myeloid leukemia |
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| Relations |
| BioProject |
PRJNA805171 |
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