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Series GSE196479 Query DataSets for GSE196479
Status Public on Jan 30, 2023
Title Mutant NPM1 binds chromatin and directly regulates oncogenic transcription in acute myeloid leukemia [SLAM-seq]
Organism Homo sapiens
Experiment type Other
Summary We use an endogenously degrader tagged NPM1c leukemia cell line that allows rapid small molecule induced degradation to show that NPM1c occupies specific chromatin targets in AML.
 
Overall design To investigate rapid changes just minutes after NPM1c degradation, we performed nascent mRNA sequencing (SLAM-seq) in NPM1 mutant leukemia cell line (OCI-AML3-NPM1c-FKBP12) in which only the mutant allele of NPM1 is modified to produce an endogenously tagged version of NPM1c that has an FKBP12 tag treated with dTAG-13 for 15 to 180 minutes.
 
Contributor(s) Uckelmann H, Hatton C
Citation(s) 36455613
Submission date Feb 10, 2022
Last update date Jan 30, 2023
Contact name Charlie Hatton
E-mail(s) Charles_Hatton@dfci.harvard.edu
Organization name Dana-Farber Cancer Institute
Department Pediatric Oncology
Lab Scott Armstrong/CPCT
Street address 450 Brookline Ave
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (18)
GSM5884996 OCIAML3-NPM1c-FKBP_SLAM_DMSO_0m_REP1_210819_CAGCGT_S1
GSM5884997 OCIAML3-NPM1c-FKBP_SLAM_DMSO_0m_REP2_210819_GATCAC_S2
GSM5884998 OCIAML3-NPM1c-FKBP_SLAM_DMSO_0m_REP3_210819_ACCAGT_S3
This SubSeries is part of SuperSeries:
GSE196480 Mutant NPM1 binds chromatin and directly regulates oncogenic transcription in acute myeloid leukemia
Relations
BioProject PRJNA805170

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Supplementary file Size Download File type/resource
GSE196479_OCIAML3-NPM1c-FKBP_SLAMseq_dTAG_differential_expression_summary_all_timepoints.xlsx 17.7 Mb (ftp)(http) XLSX
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