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| Status |
Public on Oct 18, 2010 |
| Title |
Cross-platform correlation analysis of global microRNA expression technologies |
| Platform organisms |
Caenorhabditis elegans; Drosophila melanogaster; Danio rerio; Homo sapiens; Mus musculus; Rattus norvegicus; Human alphaherpesvirus 1; Human betaherpesvirus 5; Murid betaherpesvirus 1; human gammaherpesvirus 4; JC polyomavirus; Human immunodeficiency virus 1; Murid gammaherpesvirus 4; Human gammaherpesvirus 8; Betapolyomavirus hominis; Betapolyomavirus macacae |
| Sample organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
Two reference pools comprised of different miRNA content were hybridized to four different miRNA microarray platforms (Agilent, LC Sciences, Exiqon and a homemade chip (generated from an Inviteogen Ncode probeset))
This dataset includes the reference samples on the Agilent miRNA array.
Keywords: miRNA
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| Overall design |
Two reference pools were created from commercially available reference RNA (FirstChoice® mouse Total RNA including the small fraction (Catalogue # AM7800-AM7828, Ambion, Streetsville, ON)). Reference #1 contained equal parts of mouse testicle, ovary and 10-12 day embryo. Reference #2 contained liver, heart and lung. These references were hybridized to four different miRNA microarray platforms (two in two colour, two in one-colour, with 4 replicates each). Data from each platform were normalized by cyclic lowess and analysed by correlation analyses both within and between platforms.
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| |
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| Contributor(s) |
Yauk C, Williams A |
| Citation(s) |
20504329 |
| |
| Submission date |
Dec 28, 2009 |
| Last update date |
Jan 30, 2018 |
| Contact name |
Andrew Williams |
| Organization name |
Health Canada
|
| Street address |
50 Columbine
|
| City |
Ottawa |
| State/province |
ON |
| ZIP/Postal code |
K1A OK9 |
| Country |
Canada |
| |
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| Platforms (4)
|
| GPL7723 |
miRCURY LNA microRNA Array, v.11.0 - hsa, mmu & rno |
| GPL8530 |
LC_MRA-1002_miRMouse_13.0_090309 |
| GPL8824 |
Agilent-019119 Mouse miRNA Microarray 1.0 G4472A (miRNA ID version) |
| GPL9838 |
NCode Multi-Species miRBase Microarray V2 |
|
| Samples (24)
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| Relations |
| BioProject |
PRJNA122489 |
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