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Series GSE196869 Query DataSets for GSE196869
Status Public on Jan 06, 2023
Title The application of a reverse transcriptase inhibitor suppresses LTR retrotransposon mobilization in plants [RNA-seq]
Organism Arabidopsis thaliana
Experiment type Expression profiling by high throughput sequencing
Summary Recently developed methods applied to plant material, for example tissue culture during plant transformation, can induce genome instability by activating uncontrolled mobilization of LTR retrotransposons (LTR-TEs), the most abundant class of mobile genetic elements in plant genomes. Here we tested the use of Reverse Transcriptase inhibitors to avoid LTR-TE mobilization in plants. We demonstrated that the application of the drug Tenofovir in systems with high LTR-TE activity, like Arabidopsis and rice, allows generation of plants free from LTR-TE insertions without interfering with their development. We propose the use of Tenofovir as a new tool to both study LTR-TE transposition and to regenerate more genetically stable plant lines from tissue culture.
Overall design Transcriptional profile of Arabidopsis thaliana seedlings germinated in presence and absence of Tenofovir, analysed before stress, after heat stress and three days after recovery from the stress. Each condition has three biological replicates.
Contributor(s) Catoni M
Citation(s) 36583226
Submission date Feb 16, 2022
Last update date Jan 09, 2023
Contact name Marco Catoni
Organization name University of Birmingham
Department School of Biosciences
Street address Edgbaston
City Birmingham
ZIP/Postal code B15 2TT
Country United Kingdom
Platforms (1)
GPL19580 Illumina NextSeq 500 (Arabidopsis thaliana)
Samples (18)
GSM5903344 CC_0_A
GSM5903345 CC_0_B
GSM5903346 CC_0_C
BioProject PRJNA807632

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE196869_RAW.tar 1.3 Gb (http)(custom) TAR (of BW)
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Raw data are available in SRA
Processed data provided as supplementary file

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