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Series GSE197581 Query DataSets for GSE197581
Status Public on Jul 01, 2022
Title Clock controlled and cold induced CDF6 alters growth and development to changing temperatures
Organism Arabidopsis thaliana
Experiment type Expression profiling by high throughput sequencing
Summary The circadian clock represents a critical regulatory network, which allows plants to anticipate environmental changes as inputs and promote plant survival by regulating various physiological outputs. Here, we examine the function of the clock-regulated transcription factor, CYCLING DOF FACTOR 6 (CDF6), during cold stress in Arabidopsis thaliana. We found that the clock gates CDF6 transcript accumulation in the vasculature during cold stress. CDF6 mis-expression results in an altered flowering phenotype during both ambient and cold stress. A genome-wide transcriptome analysis links CDF6 to genes associated with flowering and seed germination during cold and ambient temperatures, respectively. Analysis of key floral regulators indicates that CDF6 alters flowering during cold stress by repressing photoperiodic flowering components, FLOWERING LOCUS T (FT), CONSTANS (CO), and BROTHER OF FT (BFT). Gene ontology enrichment further suggests that CDF6 regulates circadian and developmental associated genes. These results provide insight into how the clock-controlled CDF6 modulates plant development during moderate cold stress.
Overall design Measured transciptome changes in Columbia-0 (WT), cdf6, and SUC2::CDF6 under ambient (22°C) and 1-hr cold (10°C) temperature on 11-day-old seedlings entrained in diurnal light for 8 days before transfer to constant light for two days. Three replicates were sampled for all genotypes and conditions at ZT0.
Contributor(s) Blair EJ, Goralogia GS, Lincoln MJ, Imaizumi T, Nagel DH
Citation(s) 35958204
Submission date Feb 28, 2022
Last update date Aug 24, 2022
Contact name Dawn Nagel
Organization name University of Califonia, Riverside
Department Botany and Plant Sciences
Lab Dawn Nagel
Street address 3401 Watkins Avenue (Batchelor Hall Rm 1123)
City Riverside
State/province CA
ZIP/Postal code 92507
Country USA
Platforms (1)
GPL19580 Illumina NextSeq 500 (Arabidopsis thaliana)
Samples (18)
GSM5920861 Seedlings, WT, 10, Rep1
GSM5920862 Seedlings, WT, 10, Rep2
GSM5920863 Seedlings, WT, 10, Rep3
BioProject PRJNA811122

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE197581_Processed_Data.xlsx 14.2 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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