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Series GSE19768 Query DataSets for GSE19768
Status Public on Oct 31, 2013
Title UV-B regulation of microRNAs in maize
Platform organisms Physcomitrium patens; Pinus taeda; Gossypium hirsutum; Populus trichocarpa; Arabidopsis thaliana; Brassica napus; Glycine max; Medicago truncatula; Solanum lycopersicum; Oryza sativa; Saccharum officinarum; Sorghum bicolor; Triticum aestivum; Zea mays; Vitis vinifera; Selaginella moellendorffii
Sample organism Zea mays
Experiment type Non-coding RNA profiling by array
Summary Differential expression of microRNAs was studied in maize leaves after an 8-h-exposure under UV-B light. As a control, plants were kept in the greenhouse in the absence of UV-B
 
Overall design 4-week maize plants were grown in the greenhouse in the absence of UV-B. Then, they were divided in two groups. One group was treated with UV-B lights provided once for 8 h, starting 3 h after the beginning of the light period, using fixtures mounted 30 cm above the plants (Phillips, F40UVB 40 W and TL 20 W/12) at a UV-B intensity of 2 W m-2, UV-A: 0.65 W m-2. The bulbs were covered with cellulose acetate to exclude wavelengths <280 nm. For the second control group, plants were exposed for 8 h under the same lamps covered with polyester film (no UV-B treatment, UV-B: 0.04 W m-2, UV-A: 0.4 W m-2). Lamp output was recorded using a UV-B/UV-A radiometer (UV203 A+B radiometer, Macam Photometrics, Ltd, Livingston, UK) to insure that both the bulbs and filters provided the designated UV dosage in all treatments. Leaf samples were collected immediately after irradiation. RNA was extracted immediately after the treatments, and used for the microRNA microarray experiments.
 
Contributor(s) Casati P
Citation(s) 24494230
Submission date Jan 06, 2010
Last update date Sep 12, 2014
Contact name Paula Casati
E-mail(s) casati@cefobi-conicet.gov.ar
Phone 54-341-4371955
Organization name Universidad Nacional de Rosario
Department Biological Sciences
Lab Casati
Street address Suipacha 531
City Rosario
State/province Santa Fe
ZIP/Postal code 2000
Country Argentina
 
Platforms (1)
GPL9858 MiRPlants-12.0 plus 100 maize smRNAs
Samples (2)
GSM493629 UV-B regulation of microRNAs in maize
GSM493630 UV-B regulation of microRNAs in maize-2
Relations
BioProject PRJNA121849

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE19768_RAW.tar 200.0 Kb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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