 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Apr 01, 2022 |
| Title |
Functional reprogramming of monocytes in acute and convalescent severe COVID-19 patients [ATAC-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Severe COVID-19 disease is associated with dysregulation of the myeloid compartment during acute infection. Survivors frequently experience long-lasting sequelae but little is known about the eventual persistence of this immune alteration. Herein, we evaluated Toll-like receptor-induced cytokine responses in a cohort of mild to critical patients during acute or convalescent phases (n=97). In the acute phase, we observed impaired cytokine production by monocytes in the most severe patients. This capacity was globally restored in convalescent patients. Yet, we observed increased responsiveness to TLR1/2 ligation in patients that recovered from severe disease, indicating that these cells display distinct functional properties at the different stages of the disease. We identified a specific transcriptomic and epigenomic state in monocytes from acute severe patients that can account for their functional refractoriness. The molecular profile of monocytes from recovering patients was distinct and characterized by increased chromatin accessibility at AP-1 and MAF loci. These results demonstrate that severe COVID-19 infection has a profound impact on the differentiation status and function of circulating monocytes both during the acute and the convalescent phases in a completely distinct manner. This could have important implications for our understanding of short and long-term COVID19-related morbidity.
|
| |
|
| Overall design |
ATAC-seq
|
| |
|
| Contributor(s) |
Brauns E, Azouz A, Grimaldi D, Xiao H, Thomas S, Nguyen M, Olislagers V, Vu Duc I, Orte Cano C, Del Marmol V, Pannus P, Libert F, Saussez S, Dauby N, Das J, Marchant A, Goriely S |
| Citation(s) |
35380990 |
| |
| Submission date |
Mar 09, 2022 |
| Last update date |
May 25, 2022 |
| Contact name |
Abdulkader AZOUZ |
| E-mail(s) |
Abdulkader.azouz@ulb.be
|
| Organization name |
Université Libre de Bruxelles
|
| Street address |
Rue Adrienne Bolland, 8
|
| City |
GOSSELIES |
| ZIP/Postal code |
6041 |
| Country |
Belgium |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (30)
|
|
| This SubSeries is part of SuperSeries: |
| GSE198257 |
Functional reprogramming of monocytes in acute and convalescent severe COVID-19 patients |
|
| Relations |
| BioProject |
PRJNA814291 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE198255_Acute.bw |
326.7 Mb |
(ftp)(http) |
BW |
| GSE198255_Acute_vs_control_significant.txt.gz |
167.7 Kb |
(ftp)(http) |
TXT |
| GSE198255_HC.bw |
325.9 Mb |
(ftp)(http) |
BW |
| GSE198255_Recovery3Mo.bw |
287.5 Mb |
(ftp)(http) |
BW |
| GSE198255_Recovery6Mo.bw |
325.4 Mb |
(ftp)(http) |
BW |
| GSE198255_Recovery_3Mo_vs_control_significant.txt.gz |
271.2 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...