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Series GSE198255 Query DataSets for GSE198255
Status Public on Apr 01, 2022
Title Functional reprogramming of monocytes in acute and convalescent severe COVID-19 patients [ATAC-seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Severe COVID-19 disease is associated with dysregulation of the myeloid compartment during acute infection. Survivors frequently experience long-lasting sequelae but little is known about the eventual persistence of this immune alteration. Herein, we evaluated Toll-like receptor-induced cytokine responses in a cohort of mild to critical patients during acute or convalescent phases (n=97). In the acute phase, we observed impaired cytokine production by monocytes in the most severe patients. This capacity was globally restored in convalescent patients. Yet, we observed increased responsiveness to TLR1/2 ligation in patients that recovered from severe disease, indicating that these cells display distinct functional properties at the different stages of the disease. We identified a specific transcriptomic and epigenomic state in monocytes from acute severe patients that can account for their functional refractoriness. The molecular profile of monocytes from recovering patients was distinct and characterized by increased chromatin accessibility at AP-1 and MAF loci. These results demonstrate that severe COVID-19 infection has a profound impact on the differentiation status and function of circulating monocytes both during the acute and the convalescent phases in a completely distinct manner. This could have important implications for our understanding of short and long-term COVID19-related morbidity.
Overall design ATAC-seq
Contributor(s) Brauns E, Azouz A, Grimaldi D, Xiao H, Thomas S, Nguyen M, Olislagers V, Vu Duc I, Orte Cano C, Del Marmol V, Pannus P, Libert F, Saussez S, Dauby N, Das J, Marchant A, Goriely S
Citation(s) 35380990
Submission date Mar 09, 2022
Last update date May 25, 2022
Contact name Abdulkader AZOUZ
Organization name Université Libre de Bruxelles
Street address Rue Adrienne Bolland, 8
ZIP/Postal code 6041
Country Belgium
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (30)
GSM5942309 ICOV1-017
GSM5942310 COVSP005
GSM5942311 ICOV6-013
This SubSeries is part of SuperSeries:
GSE198257 Functional reprogramming of monocytes in acute and convalescent severe COVID-19 patients
BioProject PRJNA814291

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource 326.7 Mb (ftp)(http) BW
GSE198255_Acute_vs_control_significant.txt.gz 167.7 Kb (ftp)(http) TXT 325.9 Mb (ftp)(http) BW 287.5 Mb (ftp)(http) BW 325.4 Mb (ftp)(http) BW
GSE198255_Recovery_3Mo_vs_control_significant.txt.gz 271.2 Kb (ftp)(http) TXT
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Processed data are available on Series record

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