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Series GSE19842 Query DataSets for GSE19842
Status Public on Dec 31, 2010
Title The effect of IAN on E. coli O157:H7 EHEC biofilm formation
Organism Escherichia coli
Experiment type Expression profiling by array
Summary Intercellular signal indole and its derivative hydroxyindoles inhibit Escherichia coli biofilm and diminish Pseudomonas aeruginosa virulence. However, indole and bacterial indole derivatives were unstable in microbial community due to the widespread of diverse oxygenases that could quickly degrade them. Hence, we sought to identify novel non-toxic, stable, and potent indole derivatives from plant sources for inhibiting biofilm formation of E. coli O157:H7 and P. aeruginosa PAO1. Here, plant auxin 3-indolylacetonitrile (IAN) was found to inhibit biofilm formation of both E. coli O157:H7 and P. aeruginosa without affecting its growth. IAN inhibited biofilms more effectively than indole for both E. coli and P. aeruginosa. Additionally, IAN decreased the production of virulence factor pyocyanin in P. aeruginosa. DNA microarray analysis indicated that IAN repressed genes involved in curli formation and glycerol metabolism, while IAN induced indole-related genes and prophage genes in E. coli. It appears that IAN inhibits biofilm formation of E. coli by reducing curli formation and inducing indole production. Furthermore, unlike bacterial indole derivatives, plant-originated IAN was stable in the presence of either E. coli or P. aeruginosa.
Overall design For the microarray experiments, E. coli O157:H7 were inoculated in 25 ml of LB medium in 250 ml shake flasks with overnight cultures that were diluted 1:100. IAN (100 μg/ml) dissolved in 25 μl DMSO or 25 μl DMSO alone as a control was added. Cells were cultured in LB at 37C with 250 rpm shaking until an absorbance of 1.0 at 600 nm. Cells were immediately chilled with dry ice and 95% ethanol (to prevent RNA degradation) for 30 sec before centrifugation in 50 ml centrifuge tubes at 13,000 g for 2 min; cell pellets were frozen immediately with dry ice and stored -80°C. RNA was isolated using Qiagen RNeasy mini Kit (Valencia, CA, USA). RNA quality was assessed by Agilent 2100 bioanalyser using the RNA 6000 Nano Chip (Agilent Technologies, Amstelveen, The Netherlands), and quantity was determined by ND-1000 Spectrophotometer (NanoDrop Technologies, Inc., DE, USA).
Contributor(s) Lee J, Lee J
Citation(s) 20649646
Submission date Jan 12, 2010
Last update date Mar 08, 2019
Contact name Jintae Lee
Phone 82-53-810-2533
Organization name Yeungnam University
Department Chemical engineering
Lab Biotechnology
Street address 214-1 Daedong
City Gyeongsan-Si
State/province Gyeongsangbuk-Do
ZIP/Postal code 712-749
Country South Korea
Platforms (1)
GPL3154 [E_coli_2] Affymetrix E. coli Genome 2.0 Array
Samples (2)
GSM495718 EDL 933 at 37oC at OD 1.0
GSM495719 EDL 933 with 0.1 mg/ml IAN at 37oC at OD 1.0
BioProject PRJNA122065

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE19842_RAW.tar 2.0 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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