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Series GSE198669 Query DataSets for GSE198669
Status Public on Mar 13, 2023
Title Longitudinal transcriptomic analysis of mouse sciatic nerve reveals pathways associated with age-related muscle pathology
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Our objective was to identify early changes in gene expression related to the development of sarcopenia via transcriptomic profiling of aging sciatic nerves in vivo. Sciatic nerve mRNA profiles of C57BL/6JN mice aged 5 (n = 6), 18 (n = 5), 21 (n = 6), and 24 months (n = 5) old were generated by RNA sequencing using an Illumina HiSeq4000. The sequence reads that passed quality filters were mapped to the mouse reference genome using STAR and featureCounts. Differential expression analysis was performed using DESeq2. Differentially expressed genes were validated using quantitative reverse transcription PCR (qRT-PCR) using SYBR Green assays.

We detected 51 significant differentially expressed genes in sciatic nerve of 18-month-old mice compared to 5-month-old mice. These genes were associated with the AMPK signaling pathway, biosynthesis and metabolic pathways, and circadian rhythm. These early molecular changes shed a new light on biological processes in peripheral nerve that may be implicated in sarcopenia initiation and pathogenesis. Future studies are warranted to confirm the disease modifying and/or biomarker potential of the key changes we report here.
 
Overall design Sciatic nerve mRNA profiles compared between C57BL/6JN mice aged 5 (n = 6), 18 (n = 5), 21 (n = 6), and 24 months (n = 5).
Web link https://doi.org/10.1002/jcsm.13204
 
Contributor(s) Comfort N, Re DB
Citation(s) 36905126
Submission date Mar 15, 2022
Last update date Mar 13, 2023
Contact name Diane Berengere Re
E-mail(s) dr2240@cumc.columbia.edu
Organization name Columbia University
Department Environmental Health Sciences
Street address 630 West 168th Street
City New York
State/province New York
ZIP/Postal code 10032
Country USA
 
Platforms (1)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
Samples (24)
GSM5954712 Sciatic nerve 18 month WT rep1 [SK01]
GSM5954713 Sciatic nerve 18 month WT rep2 [SK02]
GSM5954714 Sciatic nerve 18 month WT rep3 [SK03]
Relations
BioProject PRJNA816426

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Supplementary file Size Download File type/resource
GSE198669_counts_table_raw.txt.gz 823.5 Kb (ftp)(http) TXT
GSE198669_normalized_counts.csv.gz 2.1 Mb (ftp)(http) CSV
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