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| Status |
Public on Mar 22, 2022 |
| Title |
Acute GenX exposure induces spinal deformations and arrhythmia, and alters expression of genes involved in cardiac, vascular, neural, and visual systems during zebrafish (Danio rerio) early development |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
This study assessed the potential for GenX exposure to exert adverse effects during early development of zebrafish using morphological and physiological assessments, and further performed gene expression analysis on sublethal exposures to evaluate the underlying toxicogenomics. Zebrafish embryos exposed to GenX concentrations of 0.5 – 20000 mg/L were assessed at 24, 48, and 72 hours post-fertilization (hpf) for morphology, physiology, and survival. Shallow RNA-sequencing analysis of 9465 transcripts identified 38 genes that were consistently differentially expressed at 0.5, 1, 2, and 10 mg/L GenX exposures (compared to control at 72 hpf). Downregulated genes are associated with neurotransmission (6 genes) and visual response (10 genes), and upregulated genes are expressed in or regulate the cardiovascular system (7 genes). Our results suggest acute adverse effects by the alternative PFAS, GenX, in early zebrafish development by altering expression of genes involved in or regulating the heart and vascular tissue, neurotransmission, and visual response.
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| Overall design |
Examination of effects on GenX on zebrafish embryos.
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| Contributor(s) |
Gong S, McLamb F, Shea D, Vu JP, Vasquez MF, Feng Z, Bozinovic K, Hirata K, Gersberg RM, Bozinovic G |
| Citation(s) |
36462083 |
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| Submission date |
Mar 18, 2022 |
| Last update date |
Jul 14, 2023 |
| Contact name |
Goran Bozinovic |
| E-mail(s) |
goran@bozinstitute.org
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| Phone |
19196073155
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| Organization name |
Boz Life Science Research and Teaching Institute
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| Lab |
Boz
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| Street address |
3030 Bunker Hill St
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| City |
San Diego |
| State/province |
CA |
| ZIP/Postal code |
92109-5754 |
| Country |
USA |
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| Platforms (1) |
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| Samples (20)
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| GSM5961897 |
zfish, embryo, 72hpf, 05d |
| GSM5961898 |
zfish, embryo, 72hpf, 10a |
| GSM5961899 |
zfish, embryo, 72hpf, 10b |
| GSM5961900 |
zfish, embryo, 72hpf, 10c |
| GSM5961901 |
zfish, embryo, 72hpf, 10d |
| GSM5961902 |
zfish, embryo, 72hpf, 1a |
| GSM5961903 |
zfish, embryo, 72hpf, 1b |
| GSM5961904 |
zfish, embryo, 72hpf, 1c |
| GSM5961905 |
zfish, embryo, 72hpf, 1d |
| GSM5961906 |
zfish, embryo, 72hpf, 2a |
| GSM5961907 |
zfish, embryo, 72hpf, 2b |
| GSM5961908 |
zfish, embryo, 72hpf, 2c |
| GSM5961909 |
zfish, embryo, 72hpf, 2d |
| GSM5961910 |
zfish, embryo, 72hpf, controla |
| GSM5961911 |
zfish, embryo, 72hpf, controlb |
| GSM5961912 |
zfish, embryo, 72hpf, controlc |
| GSM5961913 |
zfish, embryo, 72hpf, controld |
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| Relations |
| BioProject |
PRJNA817608 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE198976_fish_outlierrm_tmm.csv.gz |
409.2 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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